Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital

Jung Hun Lee, Ha Il Jung, Jun Ho Jung, Jin Seo Park, Jun Bae Ahn, Seok Hoon Jeong, Byeong Chul Jeong, Jung Hyun Lee, Sang Hee Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To determine dissemination and genotype of AmpC β-lactamases and an extended-spectrum β-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type β-lactamase, CMY-10. Five strains also produced one or more other β-lactamases: SHV-12, an extended-spectrum β-lactamase (five isolates); TEM-1, a class A β-lactamase (two isolates); and a chromosomal AmpC β-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the β-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum β-lactams in Korea. CMY-10 β-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from blaCMY-10 gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the blaCMY-10 gene. The blaCMY-10 gene might be inserted into a sull-type complex integron by I-15 or I-18.

Original languageEnglish
Pages (from-to)224-230
Number of pages7
JournalMicrobial Drug Resistance
Volume10
Issue number3
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Cephamycins
Integrons
Nucleotides
Korea
Genes
Plasmids
Cefotetan
Enterobacter aerogenes
Cephalothin
Amoxicillin-Potassium Clavulanate Combination
Cefoxitin
Lactams
Polymerase Chain Reaction
Amoxicillin
Enterobacteriaceae
DNA Sequence Analysis
Disease Outbreaks
Genotype
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

Cite this

Lee, Jung Hun ; Jung, Ha Il ; Jung, Jun Ho ; Park, Jin Seo ; Ahn, Jun Bae ; Jeong, Seok Hoon ; Jeong, Byeong Chul ; Lee, Jung Hyun ; Lee, Sang Hee. / Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital. In: Microbial Drug Resistance. 2004 ; Vol. 10, No. 3. pp. 224-230.
@article{2950d7b5868d47efa1e054f09ef741b5,
title = "Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital",
abstract = "To determine dissemination and genotype of AmpC β-lactamases and an extended-spectrum β-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type β-lactamase, CMY-10. Five strains also produced one or more other β-lactamases: SHV-12, an extended-spectrum β-lactamase (five isolates); TEM-1, a class A β-lactamase (two isolates); and a chromosomal AmpC β-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the β-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum β-lactams in Korea. CMY-10 β-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from blaCMY-10 gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the blaCMY-10 gene. The blaCMY-10 gene might be inserted into a sull-type complex integron by I-15 or I-18.",
author = "Lee, {Jung Hun} and Jung, {Ha Il} and Jung, {Jun Ho} and Park, {Jin Seo} and Ahn, {Jun Bae} and Jeong, {Seok Hoon} and Jeong, {Byeong Chul} and Lee, {Jung Hyun} and Lee, {Sang Hee}",
year = "2004",
month = "9",
day = "1",
doi = "10.1089/mdr.2004.10.224",
language = "English",
volume = "10",
pages = "224--230",
journal = "Microbial Drug Resistance",
issn = "1076-6294",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

Lee, JH, Jung, HI, Jung, JH, Park, JS, Ahn, JB, Jeong, SH, Jeong, BC, Lee, JH & Lee, SH 2004, 'Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital', Microbial Drug Resistance, vol. 10, no. 3, pp. 224-230. https://doi.org/10.1089/mdr.2004.10.224

Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital. / Lee, Jung Hun; Jung, Ha Il; Jung, Jun Ho; Park, Jin Seo; Ahn, Jun Bae; Jeong, Seok Hoon; Jeong, Byeong Chul; Lee, Jung Hyun; Lee, Sang Hee.

In: Microbial Drug Resistance, Vol. 10, No. 3, 01.09.2004, p. 224-230.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital

AU - Lee, Jung Hun

AU - Jung, Ha Il

AU - Jung, Jun Ho

AU - Park, Jin Seo

AU - Ahn, Jun Bae

AU - Jeong, Seok Hoon

AU - Jeong, Byeong Chul

AU - Lee, Jung Hyun

AU - Lee, Sang Hee

PY - 2004/9/1

Y1 - 2004/9/1

N2 - To determine dissemination and genotype of AmpC β-lactamases and an extended-spectrum β-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type β-lactamase, CMY-10. Five strains also produced one or more other β-lactamases: SHV-12, an extended-spectrum β-lactamase (five isolates); TEM-1, a class A β-lactamase (two isolates); and a chromosomal AmpC β-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the β-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum β-lactams in Korea. CMY-10 β-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from blaCMY-10 gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the blaCMY-10 gene. The blaCMY-10 gene might be inserted into a sull-type complex integron by I-15 or I-18.

AB - To determine dissemination and genotype of AmpC β-lactamases and an extended-spectrum β-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type β-lactamase, CMY-10. Five strains also produced one or more other β-lactamases: SHV-12, an extended-spectrum β-lactamase (five isolates); TEM-1, a class A β-lactamase (two isolates); and a chromosomal AmpC β-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the β-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum β-lactams in Korea. CMY-10 β-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from blaCMY-10 gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the blaCMY-10 gene. The blaCMY-10 gene might be inserted into a sull-type complex integron by I-15 or I-18.

UR - http://www.scopus.com/inward/record.url?scp=4644340891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644340891&partnerID=8YFLogxK

U2 - 10.1089/mdr.2004.10.224

DO - 10.1089/mdr.2004.10.224

M3 - Article

C2 - 15383166

AN - SCOPUS:4644340891

VL - 10

SP - 224

EP - 230

JO - Microbial Drug Resistance

JF - Microbial Drug Resistance

SN - 1076-6294

IS - 3

ER -