Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital

Jung Hun Lee, Ha Il Jung, Jun Ho Jung, Jin Seo Park, Jun Bae Ahn, Seok Hoon Jeong, Byeong Chul Jeong, Jung Hyun Lee, Sang Hee Lee

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


To determine dissemination and genotype of AmpC β-lactamases and an extended-spectrum β-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type β-lactamase, CMY-10. Five strains also produced one or more other β-lactamases: SHV-12, an extended-spectrum β-lactamase (five isolates); TEM-1, a class A β-lactamase (two isolates); and a chromosomal AmpC β-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the β-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum β-lactams in Korea. CMY-10 β-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from blaCMY-10 gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the blaCMY-10 gene. The blaCMY-10 gene might be inserted into a sull-type complex integron by I-15 or I-18.

Original languageEnglish
Pages (from-to)224-230
Number of pages7
JournalMicrobial Drug Resistance
Issue number3
Publication statusPublished - 2004 Sept

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)


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