Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital

Jung Hun Lee, Ha Il Jung, Jun Ho Jung, Jin Seo Park, Jun Bae Ahn, Seok Hoon Jeong, Byeong Chul Jeong, Jung Hyun Lee, Sang Hee Lee

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20 Citations (Scopus)

Abstract

To determine dissemination and genotype of AmpC β-lactamases and an extended-spectrum β-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type β-lactamase, CMY-10. Five strains also produced one or more other β-lactamases: SHV-12, an extended-spectrum β-lactamase (five isolates); TEM-1, a class A β-lactamase (two isolates); and a chromosomal AmpC β-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the β-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum β-lactams in Korea. CMY-10 β-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from blaCMY-10 gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the blaCMY-10 gene. The blaCMY-10 gene might be inserted into a sull-type complex integron by I-15 or I-18.

Original languageEnglish
Pages (from-to)224-230
Number of pages7
JournalMicrobial Drug Resistance
Volume10
Issue number3
DOIs
Publication statusPublished - 2004 Sep

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

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    Lee, J. H., Jung, H. I., Jung, J. H., Park, J. S., Ahn, J. B., Jeong, S. H., Jeong, B. C., Lee, J. H., & Lee, S. H. (2004). Dissemination of transferable AmpC-type β-lactamase (CMY-10) in a Korean hospital. Microbial Drug Resistance, 10(3), 224-230. https://doi.org/10.1089/mdr.2004.10.224