Distance-mediated plasmonic dimers for reusable colorimetric switches: A measurable peak shift of more than 60 nm

Longhua Guo, Abdul Rahim Ferhan, Hailan Chen, Changming Li, Guonan Chen, Seungpyo Hong, Dong Hwan Kim

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The first reconfigurable colorimetric DNA switches based on target DNA binding are reported. This DNA binding actuates a change in the interparticle distance between gold nanoparticle dimers. A significant spectral shift of 68 nm is achievable from on-off switching. The reconfigurability is possible owing to thiol and EDC-imidazole coupling which anchors the DNA linkers to the nanoparticles. The huge spectral shift allows the unaided eye to observe single target biomolecular binding event in real time under a darkfield microscope. The limit-of-detection for target molecules in PBS and human serum are 10 -13 M and 10-11 M respectively. An improved fabrication strategy via asymmetric functionalization is also described, assisted by solid phase synthesis which minimizes the formation of trimers and multimers.

Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalSmall
Volume9
Issue number2
DOIs
Publication statusPublished - 2013 Jan 28

Fingerprint

Dimers
DNA
Switches
Nanoparticles
Solid-Phase Synthesis Techniques
Anchors
Sulfhydryl Compounds
Gold
Limit of Detection
Microscopes
Fabrication
Molecules
Serum

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Engineering (miscellaneous)

Cite this

Guo, Longhua ; Ferhan, Abdul Rahim ; Chen, Hailan ; Li, Changming ; Chen, Guonan ; Hong, Seungpyo ; Kim, Dong Hwan. / Distance-mediated plasmonic dimers for reusable colorimetric switches : A measurable peak shift of more than 60 nm. In: Small. 2013 ; Vol. 9, No. 2. pp. 234-240.
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Distance-mediated plasmonic dimers for reusable colorimetric switches : A measurable peak shift of more than 60 nm. / Guo, Longhua; Ferhan, Abdul Rahim; Chen, Hailan; Li, Changming; Chen, Guonan; Hong, Seungpyo; Kim, Dong Hwan.

In: Small, Vol. 9, No. 2, 28.01.2013, p. 234-240.

Research output: Contribution to journalArticle

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AU - Guo, Longhua

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AU - Chen, Guonan

AU - Hong, Seungpyo

AU - Kim, Dong Hwan

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AB - The first reconfigurable colorimetric DNA switches based on target DNA binding are reported. This DNA binding actuates a change in the interparticle distance between gold nanoparticle dimers. A significant spectral shift of 68 nm is achievable from on-off switching. The reconfigurability is possible owing to thiol and EDC-imidazole coupling which anchors the DNA linkers to the nanoparticles. The huge spectral shift allows the unaided eye to observe single target biomolecular binding event in real time under a darkfield microscope. The limit-of-detection for target molecules in PBS and human serum are 10 -13 M and 10-11 M respectively. An improved fabrication strategy via asymmetric functionalization is also described, assisted by solid phase synthesis which minimizes the formation of trimers and multimers.

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