Distinct and overlapping roles of single-repeat MYB genes in root epidermal patterning

Marissa Simon, Myeong Min Lee, Yan Lin, Lindsey Gish, John Schiefelbein

Research output: Contribution to journalArticlepeer-review

116 Citations (Scopus)

Abstract

Cell specification in the root epidermis of Arabidopsis generates a position-dependent pattern of root-hair cells and non-hair cells. Here we conduct a comprehensive analysis of the five members of a single-repeat R3 MYB gene family, including CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER of TRY and CPC 1, 2, and 3 (ETC1, ETC2, and ETC3), and study their role and functional relationship in root epidermal cell specification. Based on genetic and expression analyses, CPC, TRY and ETC1, but not ETC2 or ETC3, promote the hair cell fate by inhibiting non-hair specification. Further, we find that single-repeat MYB activity is required for epidermal patterning throughout root development, beginning during embryogenesis. We also identify a novel regulatory interaction whereby GLABRA2 (GL2) promotes TRY (but not CPC or ETC1) expression in the root epidermis, which generates a second lateral inhibition feedback loop. Gene fusion experiments combining CPC regulatory elements with protein-coding regions of each single-repeat MYB gene suggest that all five proteins are functionally similar, although TRY and ETC2 exhibit distinctions from CPC/ETC1/ETC3. These results provide new insight into the function of these single-repeat MYBs and suggest that divergence of their regulatory sequences is largely responsible for their distinct roles in epidermal cell patterning.

Original languageEnglish
Pages (from-to)566-578
Number of pages13
JournalDevelopmental Biology
Volume311
Issue number2
DOIs
Publication statusPublished - 2007 Nov 15

Bibliographical note

Funding Information:
We are grateful to the Salk Institute Genomic Analysis Laboratory and the Arabidopsis Biological Resource Center for the SALK 040390 ( etc2-2 ) and SALK 094027 ( etc3-1 ) insertion lines. We thank Su-Hwan Kwak, Christine Bernhardt, and Christa Barron for their helpful comments and advice. This work was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2004-35304-14924, the National Science Foundation (IBN 0316312), and the Cellular Biotechnology Training Program at the University of Michigan.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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