Distinct and redundant functions of μ1 medium chains of the ap-1 clathrin-associated protein complex in the nematode Caenorhabditis elegans

J. Shim, P. W. Sternberg, JooHun Lee

Research output: Contribution to journalArticle

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Abstract

In the nematode Caenorhabditis elegans, there exist two μ1 medium chains of the AP-1 clathrin-associated protein complex. Mutations of unc-101, the gene that encodes one of the μ1 chains, cause pleiotropic effects (Lee et al., 1994). In this report, we identified and analyzed the second μ1 chain gene, apm-1. Unlike the mammalian homologs, the two medium chains are expressed ubiquitously throughout development. RNA interference (RNAi) experiments with apm-1 showed that apm-1 and unc-101 were redundant in embryogenesis and in vulval development. Consistent with this, a hybrid protein containing APM-1, when overexpressed, rescued the phenotype of an unc-101 mutant. However, single disruptions of apm-1 or unc-101 have distinct phenotypes, indicating that the two medium chains may have distinct functions. RNAi of any one of the small or large chains of AP-1 complex (σ1, β1, or γ) showed a phenotype identical to that caused by the simultaneous disruption of unc-101 and apm-1, but not that by single disruption of either gene. This suggests that the two medium chains may share large and small chains in the AP-1 complexes. Thus, apm-1 and unc-101 encode two highly related μ1 chains that share redundant and distinct functions within AP-1 clathrin-associated protein complexes of the same tissue.

Original languageEnglish
Pages (from-to)2743-2756
Number of pages14
JournalMolecular Biology of the Cell
Volume11
Issue number8
DOIs
Publication statusPublished - 2000 Jan 1

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Vesicular Transport Adaptor Proteins
Transcription Factor AP-1
Caenorhabditis elegans
RNA Interference
Phenotype
Genes
Embryonic Development
Mutation
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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title = "Distinct and redundant functions of μ1 medium chains of the ap-1 clathrin-associated protein complex in the nematode Caenorhabditis elegans",
abstract = "In the nematode Caenorhabditis elegans, there exist two μ1 medium chains of the AP-1 clathrin-associated protein complex. Mutations of unc-101, the gene that encodes one of the μ1 chains, cause pleiotropic effects (Lee et al., 1994). In this report, we identified and analyzed the second μ1 chain gene, apm-1. Unlike the mammalian homologs, the two medium chains are expressed ubiquitously throughout development. RNA interference (RNAi) experiments with apm-1 showed that apm-1 and unc-101 were redundant in embryogenesis and in vulval development. Consistent with this, a hybrid protein containing APM-1, when overexpressed, rescued the phenotype of an unc-101 mutant. However, single disruptions of apm-1 or unc-101 have distinct phenotypes, indicating that the two medium chains may have distinct functions. RNAi of any one of the small or large chains of AP-1 complex (σ1, β1, or γ) showed a phenotype identical to that caused by the simultaneous disruption of unc-101 and apm-1, but not that by single disruption of either gene. This suggests that the two medium chains may share large and small chains in the AP-1 complexes. Thus, apm-1 and unc-101 encode two highly related μ1 chains that share redundant and distinct functions within AP-1 clathrin-associated protein complexes of the same tissue.",
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Distinct and redundant functions of μ1 medium chains of the ap-1 clathrin-associated protein complex in the nematode Caenorhabditis elegans. / Shim, J.; Sternberg, P. W.; Lee, JooHun.

In: Molecular Biology of the Cell, Vol. 11, No. 8, 01.01.2000, p. 2743-2756.

Research output: Contribution to journalArticle

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