Distinct Protein Expression Profiles of Solid-Pseudopapillary Neoplasms of the Pancreas

Minhee Park, Jong Sun Lim, Hyoung Joo Lee, Keun Na, Min Jung Lee, ChangMoo Kang, Young-Ki Paik, Hoguen Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Solid-pseudopapillary neoplasm (SPN) is an uncommon pancreatic tumor with mutation in CTNNB1 and distinct clinical and pathological features. We compared the proteomic profiles of SPN to mRNA expression. Pooled SPNs and pooled non-neoplastic pancreatic tissues were examined with high-resolution mass spectrometry. We identified 329 (150 up-regulated and 179 down-regulated) differentially expressed proteins in SPN. We identified 191 proteins (58.1% of the 329 dysregulated proteins) with the same expression tendencies in SPN based on mRNA data. Many overexpressed proteins were related to signaling pathways known to be activated in SPNs. We found that several proteins involved in Wnt signaling, including DKK4 and β-catenin, and proteins that bind β-catenin, such as FUS and NONO, were up-regulated in SPNs. Molecules involved in glycolysis, including PKM2, ENO2, and HK1, were overexpressed in accordance to their mRNA levels. In summary, SPN showed (1) distinct protein expression changes that correlated with mRNA expression, (2) overexpression of Wnt signaling proteins and proteins that bind directly to β-catenin, and (3) overexpression of proteins involved in metabolism. These findings may help develop early diagnostic biomarkers and molecular targets.

Original languageEnglish
Pages (from-to)3007-3014
Number of pages8
JournalJournal of Proteome Research
Volume14
Issue number8
DOIs
Publication statusPublished - 2015 Aug 7

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Pancreatic Neoplasms
Catenins
Proteins
Neoplasms
Messenger RNA
Wnt Proteins
Molecular Pathology
Glycolysis
Biomarkers
Proteomics
Metabolism
Mass Spectrometry
Mass spectrometry
Tumors
Tissue
Mutation
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

Park, Minhee ; Lim, Jong Sun ; Lee, Hyoung Joo ; Na, Keun ; Lee, Min Jung ; Kang, ChangMoo ; Paik, Young-Ki ; Kim, Hoguen. / Distinct Protein Expression Profiles of Solid-Pseudopapillary Neoplasms of the Pancreas. In: Journal of Proteome Research. 2015 ; Vol. 14, No. 8. pp. 3007-3014.
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abstract = "Solid-pseudopapillary neoplasm (SPN) is an uncommon pancreatic tumor with mutation in CTNNB1 and distinct clinical and pathological features. We compared the proteomic profiles of SPN to mRNA expression. Pooled SPNs and pooled non-neoplastic pancreatic tissues were examined with high-resolution mass spectrometry. We identified 329 (150 up-regulated and 179 down-regulated) differentially expressed proteins in SPN. We identified 191 proteins (58.1{\%} of the 329 dysregulated proteins) with the same expression tendencies in SPN based on mRNA data. Many overexpressed proteins were related to signaling pathways known to be activated in SPNs. We found that several proteins involved in Wnt signaling, including DKK4 and β-catenin, and proteins that bind β-catenin, such as FUS and NONO, were up-regulated in SPNs. Molecules involved in glycolysis, including PKM2, ENO2, and HK1, were overexpressed in accordance to their mRNA levels. In summary, SPN showed (1) distinct protein expression changes that correlated with mRNA expression, (2) overexpression of Wnt signaling proteins and proteins that bind directly to β-catenin, and (3) overexpression of proteins involved in metabolism. These findings may help develop early diagnostic biomarkers and molecular targets.",
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Distinct Protein Expression Profiles of Solid-Pseudopapillary Neoplasms of the Pancreas. / Park, Minhee; Lim, Jong Sun; Lee, Hyoung Joo; Na, Keun; Lee, Min Jung; Kang, ChangMoo; Paik, Young-Ki; Kim, Hoguen.

In: Journal of Proteome Research, Vol. 14, No. 8, 07.08.2015, p. 3007-3014.

Research output: Contribution to journalArticle

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