Distinctive expression patterns of hypoxia-inducible factor-1a and endothelial nitric oxide synthase following hypergravity exposure

Gun Yoon, Choong Sik Oh, Hyunsoo Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study was designed to examine the expression of hypoxia-inducible factor-1a (HIF-1a) and the level and activity of endothelial nitric oxide synthase (eNOS) in the hearts and livers of mice exposed to hypergravity. Hypergravity-induced hypoxia and the subsequent post-exposure reoxygenation significantly increased cardiac HIF-1a levels. Furthermore, the levels and activity of cardiac eNOS also showed significant increase immediately following hypergravity exposure and during the reoxygenation period. In contrast, the expression of phosphorylated Akt (p-Akt) and phosphorylated extracellular signal-regulated kinase (p-ERK) showed significant elevation only during the reoxygenation period. These data raise the possibility that the increase in cardiac HIF-1a expression induced by reoxygenation involves a cascade of signaling events, including activation of the Akt and ERK pathways. In the liver, HIF-1a expression was significantly increased immediately after hypergravity exposure, indicating that hypergravity exposure to causes hepatocellular hypoxia. The hypergravity-exposed livers showed significantly higher eNOS immunoreactivity than did those of control mice. Consistent with these results, significant increases in eNOS activity and nitrate/nitrite levels were also observed. These findings suggest that hypergravity-induced hypoxia plays a significant role in the upregulation of hepatic eNOS.

Original languageEnglish
Pages (from-to)33675-33688
Number of pages14
JournalOncotarget
Volume7
Issue number23
DOIs
Publication statusPublished - 2016 Jun 7

Fingerprint

Hypergravity
Nitric Oxide Synthase Type III
Liver
MAP Kinase Signaling System
Extracellular Signal-Regulated MAP Kinases
Nitrites
Hypoxia
Nitrates
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

@article{a3c08448b23744ce938da81fd5f7066b,
title = "Distinctive expression patterns of hypoxia-inducible factor-1a and endothelial nitric oxide synthase following hypergravity exposure",
abstract = "This study was designed to examine the expression of hypoxia-inducible factor-1a (HIF-1a) and the level and activity of endothelial nitric oxide synthase (eNOS) in the hearts and livers of mice exposed to hypergravity. Hypergravity-induced hypoxia and the subsequent post-exposure reoxygenation significantly increased cardiac HIF-1a levels. Furthermore, the levels and activity of cardiac eNOS also showed significant increase immediately following hypergravity exposure and during the reoxygenation period. In contrast, the expression of phosphorylated Akt (p-Akt) and phosphorylated extracellular signal-regulated kinase (p-ERK) showed significant elevation only during the reoxygenation period. These data raise the possibility that the increase in cardiac HIF-1a expression induced by reoxygenation involves a cascade of signaling events, including activation of the Akt and ERK pathways. In the liver, HIF-1a expression was significantly increased immediately after hypergravity exposure, indicating that hypergravity exposure to causes hepatocellular hypoxia. The hypergravity-exposed livers showed significantly higher eNOS immunoreactivity than did those of control mice. Consistent with these results, significant increases in eNOS activity and nitrate/nitrite levels were also observed. These findings suggest that hypergravity-induced hypoxia plays a significant role in the upregulation of hepatic eNOS.",
author = "Gun Yoon and Oh, {Choong Sik} and Hyunsoo Kim",
year = "2016",
month = "6",
day = "7",
doi = "10.18632/oncotarget.9372",
language = "English",
volume = "7",
pages = "33675--33688",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "23",

}

Distinctive expression patterns of hypoxia-inducible factor-1a and endothelial nitric oxide synthase following hypergravity exposure. / Yoon, Gun; Oh, Choong Sik; Kim, Hyunsoo.

In: Oncotarget, Vol. 7, No. 23, 07.06.2016, p. 33675-33688.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Distinctive expression patterns of hypoxia-inducible factor-1a and endothelial nitric oxide synthase following hypergravity exposure

AU - Yoon, Gun

AU - Oh, Choong Sik

AU - Kim, Hyunsoo

PY - 2016/6/7

Y1 - 2016/6/7

N2 - This study was designed to examine the expression of hypoxia-inducible factor-1a (HIF-1a) and the level and activity of endothelial nitric oxide synthase (eNOS) in the hearts and livers of mice exposed to hypergravity. Hypergravity-induced hypoxia and the subsequent post-exposure reoxygenation significantly increased cardiac HIF-1a levels. Furthermore, the levels and activity of cardiac eNOS also showed significant increase immediately following hypergravity exposure and during the reoxygenation period. In contrast, the expression of phosphorylated Akt (p-Akt) and phosphorylated extracellular signal-regulated kinase (p-ERK) showed significant elevation only during the reoxygenation period. These data raise the possibility that the increase in cardiac HIF-1a expression induced by reoxygenation involves a cascade of signaling events, including activation of the Akt and ERK pathways. In the liver, HIF-1a expression was significantly increased immediately after hypergravity exposure, indicating that hypergravity exposure to causes hepatocellular hypoxia. The hypergravity-exposed livers showed significantly higher eNOS immunoreactivity than did those of control mice. Consistent with these results, significant increases in eNOS activity and nitrate/nitrite levels were also observed. These findings suggest that hypergravity-induced hypoxia plays a significant role in the upregulation of hepatic eNOS.

AB - This study was designed to examine the expression of hypoxia-inducible factor-1a (HIF-1a) and the level and activity of endothelial nitric oxide synthase (eNOS) in the hearts and livers of mice exposed to hypergravity. Hypergravity-induced hypoxia and the subsequent post-exposure reoxygenation significantly increased cardiac HIF-1a levels. Furthermore, the levels and activity of cardiac eNOS also showed significant increase immediately following hypergravity exposure and during the reoxygenation period. In contrast, the expression of phosphorylated Akt (p-Akt) and phosphorylated extracellular signal-regulated kinase (p-ERK) showed significant elevation only during the reoxygenation period. These data raise the possibility that the increase in cardiac HIF-1a expression induced by reoxygenation involves a cascade of signaling events, including activation of the Akt and ERK pathways. In the liver, HIF-1a expression was significantly increased immediately after hypergravity exposure, indicating that hypergravity exposure to causes hepatocellular hypoxia. The hypergravity-exposed livers showed significantly higher eNOS immunoreactivity than did those of control mice. Consistent with these results, significant increases in eNOS activity and nitrate/nitrite levels were also observed. These findings suggest that hypergravity-induced hypoxia plays a significant role in the upregulation of hepatic eNOS.

UR - http://www.scopus.com/inward/record.url?scp=84973647177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973647177&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.9372

DO - 10.18632/oncotarget.9372

M3 - Article

VL - 7

SP - 33675

EP - 33688

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 23

ER -