Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth

Ji Hee Kim, Mijeong Jeon, Je Seon Song, Jae Ho Lee, Byung Jai Choi, Han Sung Jung, Seok Jun Moon, Pamela K. DenBesten, Seong Oh Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition, their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These suggest differences in gene expression and regulation, and in this study we compared gene-expression profiles of the human dental pulp from deciduous and permanent teeth. Pulp tissues from permanent premolars and deciduous molars aged 11-14 years were extirpated and mRNA was isolated for cDNA microarray analysis, and quantitative real-time PCR (qPCR). Other teeth were used for immunohistochemical analysis (IHC). Microarray analysis identified 263 genes with a twofold or greater difference in expression level between the two types of pulp tissue, 43 and 220 of which were more abundant in deciduous and permanent pulp tissues, respectively. qPCR analysis was conducted for eight randomly selected genes, and the findings were consistent with the cDNA microarray results. IHC confirmed that insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) was broadly expressed in deciduous dental pulp tissue, but minimally expressed in permanent dental pulp tissue. Immunohistochemical analysis showed that calbindin 1 (CALB1), leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5 ), and gamma-aminobutyric acid A receptor beta 1 (GABRB1) were abundantly expressed in permanent predentin/odontoblasts, but only minimally expressed in deciduous dental pulp tissue. These results show that deciduous and permanent pulp tissues have different characteristics and gene expression, suggesting that they may have different functions and responses to therapies focused on pulp or dentin regeneration.

Original languageEnglish
Article numbere102893
JournalPloS one
Volume9
Issue number7
DOIs
Publication statusPublished - 2014 Jul 21

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tooth pulp
Dental Pulp
Deciduous Tooth
Human Activities
Pulp
pulp
teeth
Dental Pulp Test
Tissue
Gene expression
Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
gene expression
Calbindin 1
calbindin
Insulin-Like Growth Factor Binding Protein 2
Odontoblasts
therapeutics
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kim, Ji Hee ; Jeon, Mijeong ; Song, Je Seon ; Lee, Jae Ho ; Choi, Byung Jai ; Jung, Han Sung ; Moon, Seok Jun ; DenBesten, Pamela K. ; Kim, Seong Oh. / Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth. In: PloS one. 2014 ; Vol. 9, No. 7.
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Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth. / Kim, Ji Hee; Jeon, Mijeong; Song, Je Seon; Lee, Jae Ho; Choi, Byung Jai; Jung, Han Sung; Moon, Seok Jun; DenBesten, Pamela K.; Kim, Seong Oh.

In: PloS one, Vol. 9, No. 7, e102893, 21.07.2014.

Research output: Contribution to journalArticle

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AU - Jeon, Mijeong

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AU - Lee, Jae Ho

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AU - Jung, Han Sung

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AU - DenBesten, Pamela K.

AU - Kim, Seong Oh

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