Distinguishing between apoptosis and necrosis using a capacitance sensor

Ri Mi Lee, Hyangtae Choi, Jeon Soo Shin, Kunhong Kim, Kyung-hwa Yoo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Apoptosis and necrosis are two different paths for cell death. One of differences between apoptosis and necrosis is the cell morphology. Apoptotic cells shrink without loosing the integrity of their plasma membrane and break into smaller pieces called apoptotic bodies that other body cells recognize and eat. In contrast, necrotic cells swell and their plasma membrane eventually ruptures. Since the cell membrane is closely related to the capacitance (or dielectric constant), we have fabricated a capacitance sensor, which can measure the capacitance of cells, and investigated its time dependence during apoptosis and necrosis for TE2 cells induced by TNF-related apoptosis inducing ligand (TRAIL) and ethanol. The capacitance decreases monotonically during apoptosis. For necrosis, however, step-like behaviors are observed and dips are found in the dC/dt-t curves. The time-lapse images of TE2 cells, which have been taken simultaneously with the capacitance measurements, show that the dips in the dC/dt-t curves are probably due to the rupture of cell membrane. These results suggest that apoptosis and necrosis are differentiated by the capacitance measurements.

Original languageEnglish
Pages (from-to)2586-2591
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number8
DOIs
Publication statusPublished - 2009 Apr 15

Fingerprint

Cell death
Cell membranes
Necrosis
Capacitance
Apoptosis
Sensors
Capacitance measurement
Cell Membrane
TNF-Related Apoptosis-Inducing Ligand
Rupture
Permittivity
Ethanol
Cells
Cell Death
Ligands

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Lee, Ri Mi ; Choi, Hyangtae ; Shin, Jeon Soo ; Kim, Kunhong ; Yoo, Kyung-hwa. / Distinguishing between apoptosis and necrosis using a capacitance sensor. In: Biosensors and Bioelectronics. 2009 ; Vol. 24, No. 8. pp. 2586-2591.
@article{5e3656d52fd842f68e3114e3a7fd94af,
title = "Distinguishing between apoptosis and necrosis using a capacitance sensor",
abstract = "Apoptosis and necrosis are two different paths for cell death. One of differences between apoptosis and necrosis is the cell morphology. Apoptotic cells shrink without loosing the integrity of their plasma membrane and break into smaller pieces called apoptotic bodies that other body cells recognize and eat. In contrast, necrotic cells swell and their plasma membrane eventually ruptures. Since the cell membrane is closely related to the capacitance (or dielectric constant), we have fabricated a capacitance sensor, which can measure the capacitance of cells, and investigated its time dependence during apoptosis and necrosis for TE2 cells induced by TNF-related apoptosis inducing ligand (TRAIL) and ethanol. The capacitance decreases monotonically during apoptosis. For necrosis, however, step-like behaviors are observed and dips are found in the dC/dt-t curves. The time-lapse images of TE2 cells, which have been taken simultaneously with the capacitance measurements, show that the dips in the dC/dt-t curves are probably due to the rupture of cell membrane. These results suggest that apoptosis and necrosis are differentiated by the capacitance measurements.",
author = "Lee, {Ri Mi} and Hyangtae Choi and Shin, {Jeon Soo} and Kunhong Kim and Kyung-hwa Yoo",
year = "2009",
month = "4",
day = "15",
doi = "10.1016/j.bios.2009.01.028",
language = "English",
volume = "24",
pages = "2586--2591",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "8",

}

Distinguishing between apoptosis and necrosis using a capacitance sensor. / Lee, Ri Mi; Choi, Hyangtae; Shin, Jeon Soo; Kim, Kunhong; Yoo, Kyung-hwa.

In: Biosensors and Bioelectronics, Vol. 24, No. 8, 15.04.2009, p. 2586-2591.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Distinguishing between apoptosis and necrosis using a capacitance sensor

AU - Lee, Ri Mi

AU - Choi, Hyangtae

AU - Shin, Jeon Soo

AU - Kim, Kunhong

AU - Yoo, Kyung-hwa

PY - 2009/4/15

Y1 - 2009/4/15

N2 - Apoptosis and necrosis are two different paths for cell death. One of differences between apoptosis and necrosis is the cell morphology. Apoptotic cells shrink without loosing the integrity of their plasma membrane and break into smaller pieces called apoptotic bodies that other body cells recognize and eat. In contrast, necrotic cells swell and their plasma membrane eventually ruptures. Since the cell membrane is closely related to the capacitance (or dielectric constant), we have fabricated a capacitance sensor, which can measure the capacitance of cells, and investigated its time dependence during apoptosis and necrosis for TE2 cells induced by TNF-related apoptosis inducing ligand (TRAIL) and ethanol. The capacitance decreases monotonically during apoptosis. For necrosis, however, step-like behaviors are observed and dips are found in the dC/dt-t curves. The time-lapse images of TE2 cells, which have been taken simultaneously with the capacitance measurements, show that the dips in the dC/dt-t curves are probably due to the rupture of cell membrane. These results suggest that apoptosis and necrosis are differentiated by the capacitance measurements.

AB - Apoptosis and necrosis are two different paths for cell death. One of differences between apoptosis and necrosis is the cell morphology. Apoptotic cells shrink without loosing the integrity of their plasma membrane and break into smaller pieces called apoptotic bodies that other body cells recognize and eat. In contrast, necrotic cells swell and their plasma membrane eventually ruptures. Since the cell membrane is closely related to the capacitance (or dielectric constant), we have fabricated a capacitance sensor, which can measure the capacitance of cells, and investigated its time dependence during apoptosis and necrosis for TE2 cells induced by TNF-related apoptosis inducing ligand (TRAIL) and ethanol. The capacitance decreases monotonically during apoptosis. For necrosis, however, step-like behaviors are observed and dips are found in the dC/dt-t curves. The time-lapse images of TE2 cells, which have been taken simultaneously with the capacitance measurements, show that the dips in the dC/dt-t curves are probably due to the rupture of cell membrane. These results suggest that apoptosis and necrosis are differentiated by the capacitance measurements.

UR - http://www.scopus.com/inward/record.url?scp=62949122916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62949122916&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2009.01.028

DO - 10.1016/j.bios.2009.01.028

M3 - Article

C2 - 19233636

AN - SCOPUS:62949122916

VL - 24

SP - 2586

EP - 2591

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

IS - 8

ER -