Distribution of periodontal pathogens in Korean aggressive periodontitis

Jung Wook Lee, Bong Kyu Choi, Yun Jung Yoo, Seong Ho Choi, Kyoo Sung Cho, Jung Kiu Chai, Chong Kwan Kim

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. We undertook this study to determine the prevalence of seven putative periodontopathogens in Korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. Methods: Thirty-nine aggressive periodontitis patients between 20 and 35 years old (24 males and 15 females; mean age 29.6 years) were selected according to clinical criteria. The patients were subclassified into 17 localized and 22 generalized aggressive periodontitis patients. In each of the 39 individuals, subgingival plaque samples were collected from four diseased teeth (≥6 mm probing depth, 156 sites) and one healthy site (≤3 mm probing depth, 39 sites). Polymerase chain reaction (PCR) of the 16S ribosomal RNA gene fragments (about 530 bp) of plaque bacteria and their subsequent detection by dot-blot hybridization using specific oligonucleotide probes were performed to determine the presence of seven periodontopathogens. Results: The prevalences were 75% for Actinobacillus actinomycetemcomitans, 94.2% for Tannerella forsythensis (formerly Bacteroides forsythus), 99.4% for Fusobacterium sp., 85.9% for Micromonas micros (formerly Peptostreptococcus micros), 96.8% for Porphyromonas gingivalis, 78.8% for Prevotella intermedia, and 96.8% for Treponema sp. The prevalences of these bacteria were significantly higher in diseased sites than in healthy sites. Logistic regression analysis showed that P. intermedia was more significantly associated with generalized aggressive periodontitis than the localized form, with an odds ratio of 3.28 (95% confidence interval 1.26-8.56, P=0.015). Conclusions: Our results demonstrate that the seven periodontal pathogens analyzed are strongly associated with Korean aggressive periodontitis. In particular, P. intermedia are more significantly associated with generalized aggressive periodontitis, a more severe and progressive form, than with localized aggressive periodontitis.

Original languageEnglish
Pages (from-to)1329-1335
Number of pages7
JournalJournal of Periodontology
Volume74
Issue number9
DOIs
Publication statusPublished - 2003 Sep 1

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Aggressive Periodontitis
Prevotella intermedia
Tooth Diseases
Treponema
Fusobacterium
16S Ribosomal RNA
Peptostreptococcus
Bacteria
Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Oligonucleotide Probes
rRNA Genes
Logistic Models
Odds Ratio
Regression Analysis
Confidence Intervals
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Lee, J. W., Choi, B. K., Yoo, Y. J., Choi, S. H., Cho, K. S., Chai, J. K., & Kim, C. K. (2003). Distribution of periodontal pathogens in Korean aggressive periodontitis. Journal of Periodontology, 74(9), 1329-1335. https://doi.org/10.1902/jop.2003.74.9.1329
Lee, Jung Wook ; Choi, Bong Kyu ; Yoo, Yun Jung ; Choi, Seong Ho ; Cho, Kyoo Sung ; Chai, Jung Kiu ; Kim, Chong Kwan. / Distribution of periodontal pathogens in Korean aggressive periodontitis. In: Journal of Periodontology. 2003 ; Vol. 74, No. 9. pp. 1329-1335.
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abstract = "Background: Microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. We undertook this study to determine the prevalence of seven putative periodontopathogens in Korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. Methods: Thirty-nine aggressive periodontitis patients between 20 and 35 years old (24 males and 15 females; mean age 29.6 years) were selected according to clinical criteria. The patients were subclassified into 17 localized and 22 generalized aggressive periodontitis patients. In each of the 39 individuals, subgingival plaque samples were collected from four diseased teeth (≥6 mm probing depth, 156 sites) and one healthy site (≤3 mm probing depth, 39 sites). Polymerase chain reaction (PCR) of the 16S ribosomal RNA gene fragments (about 530 bp) of plaque bacteria and their subsequent detection by dot-blot hybridization using specific oligonucleotide probes were performed to determine the presence of seven periodontopathogens. Results: The prevalences were 75{\%} for Actinobacillus actinomycetemcomitans, 94.2{\%} for Tannerella forsythensis (formerly Bacteroides forsythus), 99.4{\%} for Fusobacterium sp., 85.9{\%} for Micromonas micros (formerly Peptostreptococcus micros), 96.8{\%} for Porphyromonas gingivalis, 78.8{\%} for Prevotella intermedia, and 96.8{\%} for Treponema sp. The prevalences of these bacteria were significantly higher in diseased sites than in healthy sites. Logistic regression analysis showed that P. intermedia was more significantly associated with generalized aggressive periodontitis than the localized form, with an odds ratio of 3.28 (95{\%} confidence interval 1.26-8.56, P=0.015). Conclusions: Our results demonstrate that the seven periodontal pathogens analyzed are strongly associated with Korean aggressive periodontitis. In particular, P. intermedia are more significantly associated with generalized aggressive periodontitis, a more severe and progressive form, than with localized aggressive periodontitis.",
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Distribution of periodontal pathogens in Korean aggressive periodontitis. / Lee, Jung Wook; Choi, Bong Kyu; Yoo, Yun Jung; Choi, Seong Ho; Cho, Kyoo Sung; Chai, Jung Kiu; Kim, Chong Kwan.

In: Journal of Periodontology, Vol. 74, No. 9, 01.09.2003, p. 1329-1335.

Research output: Contribution to journalArticle

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AU - Lee, Jung Wook

AU - Choi, Bong Kyu

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N2 - Background: Microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. We undertook this study to determine the prevalence of seven putative periodontopathogens in Korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. Methods: Thirty-nine aggressive periodontitis patients between 20 and 35 years old (24 males and 15 females; mean age 29.6 years) were selected according to clinical criteria. The patients were subclassified into 17 localized and 22 generalized aggressive periodontitis patients. In each of the 39 individuals, subgingival plaque samples were collected from four diseased teeth (≥6 mm probing depth, 156 sites) and one healthy site (≤3 mm probing depth, 39 sites). Polymerase chain reaction (PCR) of the 16S ribosomal RNA gene fragments (about 530 bp) of plaque bacteria and their subsequent detection by dot-blot hybridization using specific oligonucleotide probes were performed to determine the presence of seven periodontopathogens. Results: The prevalences were 75% for Actinobacillus actinomycetemcomitans, 94.2% for Tannerella forsythensis (formerly Bacteroides forsythus), 99.4% for Fusobacterium sp., 85.9% for Micromonas micros (formerly Peptostreptococcus micros), 96.8% for Porphyromonas gingivalis, 78.8% for Prevotella intermedia, and 96.8% for Treponema sp. The prevalences of these bacteria were significantly higher in diseased sites than in healthy sites. Logistic regression analysis showed that P. intermedia was more significantly associated with generalized aggressive periodontitis than the localized form, with an odds ratio of 3.28 (95% confidence interval 1.26-8.56, P=0.015). Conclusions: Our results demonstrate that the seven periodontal pathogens analyzed are strongly associated with Korean aggressive periodontitis. In particular, P. intermedia are more significantly associated with generalized aggressive periodontitis, a more severe and progressive form, than with localized aggressive periodontitis.

AB - Background: Microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. We undertook this study to determine the prevalence of seven putative periodontopathogens in Korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. Methods: Thirty-nine aggressive periodontitis patients between 20 and 35 years old (24 males and 15 females; mean age 29.6 years) were selected according to clinical criteria. The patients were subclassified into 17 localized and 22 generalized aggressive periodontitis patients. In each of the 39 individuals, subgingival plaque samples were collected from four diseased teeth (≥6 mm probing depth, 156 sites) and one healthy site (≤3 mm probing depth, 39 sites). Polymerase chain reaction (PCR) of the 16S ribosomal RNA gene fragments (about 530 bp) of plaque bacteria and their subsequent detection by dot-blot hybridization using specific oligonucleotide probes were performed to determine the presence of seven periodontopathogens. Results: The prevalences were 75% for Actinobacillus actinomycetemcomitans, 94.2% for Tannerella forsythensis (formerly Bacteroides forsythus), 99.4% for Fusobacterium sp., 85.9% for Micromonas micros (formerly Peptostreptococcus micros), 96.8% for Porphyromonas gingivalis, 78.8% for Prevotella intermedia, and 96.8% for Treponema sp. The prevalences of these bacteria were significantly higher in diseased sites than in healthy sites. Logistic regression analysis showed that P. intermedia was more significantly associated with generalized aggressive periodontitis than the localized form, with an odds ratio of 3.28 (95% confidence interval 1.26-8.56, P=0.015). Conclusions: Our results demonstrate that the seven periodontal pathogens analyzed are strongly associated with Korean aggressive periodontitis. In particular, P. intermedia are more significantly associated with generalized aggressive periodontitis, a more severe and progressive form, than with localized aggressive periodontitis.

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