Distribution of Succinic Semialdehyde Reductase in Rat Brain

Jongeun Lee, Soo Young Choi, Jae Wook Suk, Joung Woo Hong, Byung Kwon Yoo, Eui Yul Choi, Sang Ho Jang, Kyung Ah Park, Sung Woo Cho

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Succinic semialdehyde reductase (SSR) that catalyzes the reduction of succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB) has been identified as one of the NADPH-dependent aldehyde reductases. Reduction of SSA to GHB strongly supports the proposal that GHB biosynthesis may be an important step in the GABA shunt. It is pharmacologically significant in anesthesia, evoking the state of sleep, and an increase in brain dopamine level. Monoclonal antibodies against bovine brain succinic semialdehyde reductase were produced. Using the anti-succinic semialdehyde reductase antibodies, we investigated the distribution of brain succinic semialdehyde reductase in rat brain. The brain tissues were sectioned with a basis on the rat brain atlas of Paxinos and were stained by the immunoperoxidase staining method using monoclonal antibodies. In the section of the frontal lobe, immunoreactive cells were observed in the lateral septal area, the ventral pallidum, which belongs to the substantia innominata. We could observe immunoreactive cells in the reticular thalamic nucleus, which is closely related with 'sleeping', the basal nuclei of Meynert, which is associated with Alzheimer's disease, and hypothalamic nuclei. Immunoreactive cells were also shown in raphe nuclei or the reticular formation of the midbrain, cerebellum, and inferior olivary nuclei of the medulla oblongata. Succinic semialdehyde reductase-immunoreactive cells were distributed extensively in rat brain, especially immunoreactive cells were strongly observed in the areas associated with the limbic system and reticular formation.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalMolecules and cells
Volume7
Issue number1
Publication statusPublished - 1997 Feb 28

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Brain
Substantia Innominata
Monoclonal Antibodies
Olivary Nucleus
Basal Nucleus of Meynert
Hydroxybutyrates
Septum of Brain
Aldehyde Reductase
Thalamic Nuclei
Limbic System
Medulla Oblongata
Raphe Nuclei
Reticular Formation
Atlases
Frontal Lobe
4-hydroxybutyrate dehydrogenase
NADP
Cerebellum
gamma-Aminobutyric Acid
Dopamine

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, J., Choi, S. Y., Suk, J. W., Hong, J. W., Yoo, B. K., Choi, E. Y., ... Cho, S. W. (1997). Distribution of Succinic Semialdehyde Reductase in Rat Brain. Molecules and cells, 7(1), 13-20.
Lee, Jongeun ; Choi, Soo Young ; Suk, Jae Wook ; Hong, Joung Woo ; Yoo, Byung Kwon ; Choi, Eui Yul ; Jang, Sang Ho ; Park, Kyung Ah ; Cho, Sung Woo. / Distribution of Succinic Semialdehyde Reductase in Rat Brain. In: Molecules and cells. 1997 ; Vol. 7, No. 1. pp. 13-20.
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Lee, J, Choi, SY, Suk, JW, Hong, JW, Yoo, BK, Choi, EY, Jang, SH, Park, KA & Cho, SW 1997, 'Distribution of Succinic Semialdehyde Reductase in Rat Brain', Molecules and cells, vol. 7, no. 1, pp. 13-20.

Distribution of Succinic Semialdehyde Reductase in Rat Brain. / Lee, Jongeun; Choi, Soo Young; Suk, Jae Wook; Hong, Joung Woo; Yoo, Byung Kwon; Choi, Eui Yul; Jang, Sang Ho; Park, Kyung Ah; Cho, Sung Woo.

In: Molecules and cells, Vol. 7, No. 1, 28.02.1997, p. 13-20.

Research output: Contribution to journalArticle

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AU - Lee, Jongeun

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AU - Jang, Sang Ho

AU - Park, Kyung Ah

AU - Cho, Sung Woo

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Y1 - 1997/2/28

N2 - Succinic semialdehyde reductase (SSR) that catalyzes the reduction of succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB) has been identified as one of the NADPH-dependent aldehyde reductases. Reduction of SSA to GHB strongly supports the proposal that GHB biosynthesis may be an important step in the GABA shunt. It is pharmacologically significant in anesthesia, evoking the state of sleep, and an increase in brain dopamine level. Monoclonal antibodies against bovine brain succinic semialdehyde reductase were produced. Using the anti-succinic semialdehyde reductase antibodies, we investigated the distribution of brain succinic semialdehyde reductase in rat brain. The brain tissues were sectioned with a basis on the rat brain atlas of Paxinos and were stained by the immunoperoxidase staining method using monoclonal antibodies. In the section of the frontal lobe, immunoreactive cells were observed in the lateral septal area, the ventral pallidum, which belongs to the substantia innominata. We could observe immunoreactive cells in the reticular thalamic nucleus, which is closely related with 'sleeping', the basal nuclei of Meynert, which is associated with Alzheimer's disease, and hypothalamic nuclei. Immunoreactive cells were also shown in raphe nuclei or the reticular formation of the midbrain, cerebellum, and inferior olivary nuclei of the medulla oblongata. Succinic semialdehyde reductase-immunoreactive cells were distributed extensively in rat brain, especially immunoreactive cells were strongly observed in the areas associated with the limbic system and reticular formation.

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Lee J, Choi SY, Suk JW, Hong JW, Yoo BK, Choi EY et al. Distribution of Succinic Semialdehyde Reductase in Rat Brain. Molecules and cells. 1997 Feb 28;7(1):13-20.