Diurnal regulation of cyanogenic glucoside biosynthesis and endogenous turnover in cassava

Frederik Bøgeskov Schmidt, Seok Keun Cho, Carl Erik Olsen, Seong Wook Yang, Birger Lindberg Møller, Kirsten Jørgensen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cyanogenic glucosides are present in many plants, including eudicots, monocots, and ferns and function as defence compounds based on their ability to release hydrogen cyanide. In this study, the diurnal rhythm of cyanogenic glucoside content and of transcripts and enzymes involved in their biosynthesis was monitored in cassava plants grown in a glasshouse under natural light conditions. Transcripts of CYP79D1, CYP79D2, CYP71E7/11, and UGT85K5 were at minimal levels around 9 p.m., increased during the night and decreased following onset of early morning light. Transcripts of UGT85K4 and HNL10 showed more subtle variations with a maximum reached in the afternoon. Western blots showed that the protein levels of CYP71E7/11 and UGT85K4/5 decreased during the light period to a near absence around 4 p.m. and then recovered during the dark period. Transcript and protein levels of linamarase were stable throughout the 24-hr cycle. The linamarin content increased during the dark period. In the light period, spikes in the incoming solar radiation were found to result in concomitantly reduced linamarin levels. In silico studies of the promoter regions of the biosynthetic genes revealed a high frequency of light, abiotic stress, and development-related transcription factor binding motifs. The synthesis and endogenous turnover of linamarin are controlled both at the transcript and protein levels. The observed endogenous turnover of linamarin in the light period may offer a source of reduced nitrogen to balance photosynthetic carbon fixation. The rapid decrease in linamarin content following light spikes suggests an additional function of linamarin as a ROS scavenger.

Original languageEnglish
Article numbere00038
JournalPlant Direct
Volume2
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Manihot
cassava
Biosynthesis
Glucosides
photophase
glucosides
turnover
biosynthesis
scotophase
Light
solar radiation
inflorescences
hydrogen cyanide
proteins
Liliopsida
ferns and fern allies
circadian rhythm
abiotic stress
Western blotting
transcription factors

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science

Cite this

Schmidt, F. B., Cho, S. K., Olsen, C. E., Yang, S. W., Møller, B. L., & Jørgensen, K. (2018). Diurnal regulation of cyanogenic glucoside biosynthesis and endogenous turnover in cassava. Plant Direct, 2(2), [e00038]. https://doi.org/10.1002/pld3.38
Schmidt, Frederik Bøgeskov ; Cho, Seok Keun ; Olsen, Carl Erik ; Yang, Seong Wook ; Møller, Birger Lindberg ; Jørgensen, Kirsten. / Diurnal regulation of cyanogenic glucoside biosynthesis and endogenous turnover in cassava. In: Plant Direct. 2018 ; Vol. 2, No. 2.
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Schmidt, FB, Cho, SK, Olsen, CE, Yang, SW, Møller, BL & Jørgensen, K 2018, 'Diurnal regulation of cyanogenic glucoside biosynthesis and endogenous turnover in cassava', Plant Direct, vol. 2, no. 2, e00038. https://doi.org/10.1002/pld3.38

Diurnal regulation of cyanogenic glucoside biosynthesis and endogenous turnover in cassava. / Schmidt, Frederik Bøgeskov; Cho, Seok Keun; Olsen, Carl Erik; Yang, Seong Wook; Møller, Birger Lindberg; Jørgensen, Kirsten.

In: Plant Direct, Vol. 2, No. 2, e00038, 01.02.2018.

Research output: Contribution to journalArticle

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AU - Schmidt, Frederik Bøgeskov

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AB - Cyanogenic glucosides are present in many plants, including eudicots, monocots, and ferns and function as defence compounds based on their ability to release hydrogen cyanide. In this study, the diurnal rhythm of cyanogenic glucoside content and of transcripts and enzymes involved in their biosynthesis was monitored in cassava plants grown in a glasshouse under natural light conditions. Transcripts of CYP79D1, CYP79D2, CYP71E7/11, and UGT85K5 were at minimal levels around 9 p.m., increased during the night and decreased following onset of early morning light. Transcripts of UGT85K4 and HNL10 showed more subtle variations with a maximum reached in the afternoon. Western blots showed that the protein levels of CYP71E7/11 and UGT85K4/5 decreased during the light period to a near absence around 4 p.m. and then recovered during the dark period. Transcript and protein levels of linamarase were stable throughout the 24-hr cycle. The linamarin content increased during the dark period. In the light period, spikes in the incoming solar radiation were found to result in concomitantly reduced linamarin levels. In silico studies of the promoter regions of the biosynthetic genes revealed a high frequency of light, abiotic stress, and development-related transcription factor binding motifs. The synthesis and endogenous turnover of linamarin are controlled both at the transcript and protein levels. The observed endogenous turnover of linamarin in the light period may offer a source of reduced nitrogen to balance photosynthetic carbon fixation. The rapid decrease in linamarin content following light spikes suggests an additional function of linamarin as a ROS scavenger.

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