TY - JOUR
T1 - Diversified sequences of peptide epitope for same-RNA recognition
AU - Kim, Sunghoon
AU - De Pouplana, Lluis Ribas
AU - Schimmel, Paul
PY - 1993/11/1
Y1 - 1993/11/1
N2 - We replaced an essential RNA-binding, 30-amino acid helix-loop in an Escherichia coli tRNA synthetase with an inactive and simplified "generic" sequence having 23 of the 30 amino acids as alanine and serine. Wild-type residues were restored in random combinations to generate a library with a sequence complexity of about 1.9 × 107. Active molecules were obtained by genetic selection at a frequency of ≈1% and contained variants with as many as 11 alanine/serine replacements and a total of 17 alanine/serine residues. These variants have activities which are thermodynamically competitive with that of the native protein and therefore are functionally and, most likely, conformationally equivalent.
AB - We replaced an essential RNA-binding, 30-amino acid helix-loop in an Escherichia coli tRNA synthetase with an inactive and simplified "generic" sequence having 23 of the 30 amino acids as alanine and serine. Wild-type residues were restored in random combinations to generate a library with a sequence complexity of about 1.9 × 107. Active molecules were obtained by genetic selection at a frequency of ≈1% and contained variants with as many as 11 alanine/serine replacements and a total of 17 alanine/serine residues. These variants have activities which are thermodynamically competitive with that of the native protein and therefore are functionally and, most likely, conformationally equivalent.
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U2 - 10.1073/pnas.90.21.10046
DO - 10.1073/pnas.90.21.10046
M3 - Article
C2 - 7694278
AN - SCOPUS:0027453545
VL - 90
SP - 10046
EP - 10050
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 21
ER -