Diversified sequences of peptide epitope for same-RNA recognition

Sunghoon Kim; Lluis Ribas De Pouplana; Paul Schimmel

doi:10.1073/pnas.90.21.10046

Diversified sequences of peptide epitope for same-RNA recognition

Sunghoon Kim, Lluis Ribas De Pouplana, Paul Schimmel

Department of Pharmacy

Research output: Contribution to journal › Article

16 Citations (Scopus)

Abstract

We replaced an essential RNA-binding, 30-amino acid helix-loop in an Escherichia coli tRNA synthetase with an inactive and simplified "generic" sequence having 23 of the 30 amino acids as alanine and serine. Wild-type residues were restored in random combinations to generate a library with a sequence complexity of about 1.9 × 107. Active molecules were obtained by genetic selection at a frequency of ≈1% and contained variants with as many as 11 alanine/serine replacements and a total of 17 alanine/serine residues. These variants have activities which are thermodynamically competitive with that of the native protein and therefore are functionally and, most likely, conformationally equivalent.

Original language	English
Pages (from-to)	10046-10050
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	21
DOIs	https://doi.org/10.1073/pnas.90.21.10046
Publication status	Published - 1993 Nov 1

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Kim, S., De Pouplana, L. R., & Schimmel, P. (1993). Diversified sequences of peptide epitope for same-RNA recognition. Proceedings of the National Academy of Sciences of the United States of America, 90(21), 10046-10050. https://doi.org/10.1073/pnas.90.21.10046

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10.1073/pnas.90.21.10046