Diversified sequences of peptide epitope for same-RNA recognition

Sunghoon Kim, Lluis Ribas De Pouplana, Paul Schimmel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We replaced an essential RNA-binding, 30-amino acid helix-loop in an Escherichia coli tRNA synthetase with an inactive and simplified "generic" sequence having 23 of the 30 amino acids as alanine and serine. Wild-type residues were restored in random combinations to generate a library with a sequence complexity of about 1.9 × 107. Active molecules were obtained by genetic selection at a frequency of ≈1% and contained variants with as many as 11 alanine/serine replacements and a total of 17 alanine/serine residues. These variants have activities which are thermodynamically competitive with that of the native protein and therefore are functionally and, most likely, conformationally equivalent.

Original languageEnglish
Pages (from-to)10046-10050
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number21
DOIs
Publication statusPublished - 1993 Nov 1

All Science Journal Classification (ASJC) codes

  • General

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