Diversity of ampicillin resistance genes and antimicrobial susceptibility patterns in Haemophilus influenzae strains isolated in Korea

In Suk Kim, Chang Seok Ki, Sunjoo Kim, Sup Oh Won, Ran Peck Kyong, Jae Hoon Song, Kyungwon Lee, Yong Lee Nam

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

By Etest determination of the susceptibilities of 229 Haemophilus influenzae strains isolated in Korea to 10 antibiotics, the isolates were found to be antibiotic nonsusceptible in the following order: ampicillin (58.1%), trimethoprim-sulfamethoxazole (52%), cefaclor (41.1%), clarithromycin (25.8%), chloramphenicol (14.0%), amoxicillin-clavulanic acid (13.5%), meropenem (11.7%), cefixime (10.9%), cefuroxime (9.2%), and levofloxacin (1.3%). The prevalences of each resistance class were 23.6% for β-lactamase-negative ampicillin-susceptible (BLNAS) strains; 37.6% for strains with the TEM-1 type β-lactamase gene; 1.3% for strains with the ROB-1 type β-lactamase gene; 29.3% for the β-lactamase-negative ampicillin-resistant (BLNAR) strains with a mutation in the ftsI gene, which encodes PBP 3; and 8.3% for β-lactamase-positive amoxicillin-clavulanate-resistant (BLPACR) strains, which showed both resistance mechanisms (i.e., a β-lactamase gene and a mutation in the ftsI gene). The MIC 50 s of all β-lactams, including cephem and meropenem agents, for the BLNAR strains were two to three times higher than those for the BLNAS strains. This study confirms that the prevalence of BLNAR and BLPACR strains is relatively high and for the first time confirms the presence of H. influenzae strains carrying bla ROB-1 in Korea. Even though mutations in another gene(s) might be involved in β-lactam resistance, these results suggest that mutations in the ftsI gene are important for the development of resistance to β-lactams in H. influenzae strains in Korea.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume51
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

Fingerprint

Ampicillin Resistance
Haemophilus influenzae
Korea
meropenem
Lactams
Genes
Ampicillin
Mutation
Disk Diffusion Antimicrobial Tests
Cefixime
Cefaclor
Anti-Bacterial Agents
Amoxicillin-Potassium Clavulanate Combination
Cefuroxime
Clavulanic Acid
Levofloxacin
Clarithromycin
Amoxicillin
Sulfamethoxazole Drug Combination Trimethoprim
Chloramphenicol

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Kim, In Suk ; Ki, Chang Seok ; Kim, Sunjoo ; Won, Sup Oh ; Kyong, Ran Peck ; Song, Jae Hoon ; Lee, Kyungwon ; Nam, Yong Lee. / Diversity of ampicillin resistance genes and antimicrobial susceptibility patterns in Haemophilus influenzae strains isolated in Korea. In: Antimicrobial Agents and Chemotherapy. 2007 ; Vol. 51, No. 2. pp. 453-460.
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abstract = "By Etest determination of the susceptibilities of 229 Haemophilus influenzae strains isolated in Korea to 10 antibiotics, the isolates were found to be antibiotic nonsusceptible in the following order: ampicillin (58.1{\%}), trimethoprim-sulfamethoxazole (52{\%}), cefaclor (41.1{\%}), clarithromycin (25.8{\%}), chloramphenicol (14.0{\%}), amoxicillin-clavulanic acid (13.5{\%}), meropenem (11.7{\%}), cefixime (10.9{\%}), cefuroxime (9.2{\%}), and levofloxacin (1.3{\%}). The prevalences of each resistance class were 23.6{\%} for β-lactamase-negative ampicillin-susceptible (BLNAS) strains; 37.6{\%} for strains with the TEM-1 type β-lactamase gene; 1.3{\%} for strains with the ROB-1 type β-lactamase gene; 29.3{\%} for the β-lactamase-negative ampicillin-resistant (BLNAR) strains with a mutation in the ftsI gene, which encodes PBP 3; and 8.3{\%} for β-lactamase-positive amoxicillin-clavulanate-resistant (BLPACR) strains, which showed both resistance mechanisms (i.e., a β-lactamase gene and a mutation in the ftsI gene). The MIC 50 s of all β-lactams, including cephem and meropenem agents, for the BLNAR strains were two to three times higher than those for the BLNAS strains. This study confirms that the prevalence of BLNAR and BLPACR strains is relatively high and for the first time confirms the presence of H. influenzae strains carrying bla ROB-1 in Korea. Even though mutations in another gene(s) might be involved in β-lactam resistance, these results suggest that mutations in the ftsI gene are important for the development of resistance to β-lactams in H. influenzae strains in Korea.",
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Diversity of ampicillin resistance genes and antimicrobial susceptibility patterns in Haemophilus influenzae strains isolated in Korea. / Kim, In Suk; Ki, Chang Seok; Kim, Sunjoo; Won, Sup Oh; Kyong, Ran Peck; Song, Jae Hoon; Lee, Kyungwon; Nam, Yong Lee.

In: Antimicrobial Agents and Chemotherapy, Vol. 51, No. 2, 01.02.2007, p. 453-460.

Research output: Contribution to journalArticle

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