Diversity of TEM-52 extended-spectrum β-lactamase-producing non-typhoidal Salmonella isolates in Korea

Kyungwon Lee, DongEun Yong, Jong Hwa Yum, Ho Hoon Kim, Yunsop Chong

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Objectives: Extended-spectrum β-lactamase (ESBL)-producing non-typhoidal Salmonella (NTS) isolates in Korea were characterized. Patients and methods: Five isolates of ESBL-producing NTS were isolated from stool specimens of three infants and two adults with diarrhoea. Two infants acquired the infection in the community, and three other infections were hospital acquired. Results: The isolates were one each of serovars Saintpaul, Stanley and Agona, and two Enteritidis. Cell sonicates of the isolates hydrolysed cefotaxime more efficiently than ceftazidime, and had β-lactamase bands of approximate isoelectric points 6.0 and 7.4. Sequencing revealed that the β-lactamases were TEM-52 and an OXA type. The blaOXA gene was located on a class 1 integron. Cefotaxime resistance, associated with TEM-52, was transferred by conjugation. Identical pulsed-field gel electrophoresis patterns of Xbal-digested genomic DNA were observed in initially β-lactam-susceptible serovar Agona isolates and subsequent ESBL-producing isolates from an infant, and in two isolates of serovar Enteritidis from two different patients. Conclusions: This study suggests that TEM-52-producing NTS is spreading both clonally and horizontally in Korea.

Original languageEnglish
Pages (from-to)493-496
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume52
Issue number3
DOIs
Publication statusPublished - 2003 Sep 1

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Korea
Salmonella
Cefotaxime
Integrons
Community-Acquired Infections
Lactams
Ceftazidime
Pulsed Field Gel Electrophoresis
Isoelectric Point
Cross Infection
Diarrhea
DNA
Genes
Serogroup

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology

Cite this

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title = "Diversity of TEM-52 extended-spectrum β-lactamase-producing non-typhoidal Salmonella isolates in Korea",
abstract = "Objectives: Extended-spectrum β-lactamase (ESBL)-producing non-typhoidal Salmonella (NTS) isolates in Korea were characterized. Patients and methods: Five isolates of ESBL-producing NTS were isolated from stool specimens of three infants and two adults with diarrhoea. Two infants acquired the infection in the community, and three other infections were hospital acquired. Results: The isolates were one each of serovars Saintpaul, Stanley and Agona, and two Enteritidis. Cell sonicates of the isolates hydrolysed cefotaxime more efficiently than ceftazidime, and had β-lactamase bands of approximate isoelectric points 6.0 and 7.4. Sequencing revealed that the β-lactamases were TEM-52 and an OXA type. The blaOXA gene was located on a class 1 integron. Cefotaxime resistance, associated with TEM-52, was transferred by conjugation. Identical pulsed-field gel electrophoresis patterns of Xbal-digested genomic DNA were observed in initially β-lactam-susceptible serovar Agona isolates and subsequent ESBL-producing isolates from an infant, and in two isolates of serovar Enteritidis from two different patients. Conclusions: This study suggests that TEM-52-producing NTS is spreading both clonally and horizontally in Korea.",
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Diversity of TEM-52 extended-spectrum β-lactamase-producing non-typhoidal Salmonella isolates in Korea. / Lee, Kyungwon; Yong, DongEun; Yum, Jong Hwa; Kim, Ho Hoon; Chong, Yunsop.

In: Journal of Antimicrobial Chemotherapy, Vol. 52, No. 3, 01.09.2003, p. 493-496.

Research output: Contribution to journalArticle

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