DNA analysis by flow cytometry in early gastric cancer.

J. M. Kim, D. K. Lee, Y. K. Kim, S. K. Baik, C. I. Lee, S. O. Kwon, S. H. Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVES: Flow cytometric analysis of a paraffin-embedded block of tissue provides rapid and accurate means of analyzing the DNA content of a tumor. The aim of this study was to clarify the clinical significance of flow cytometric findings in early gastric cancer(EGC). Thus we conducted this study to investigate whether DNA contents of tumor cells can correlate with known prognostic indices in patients with EGC. METHODS: The flow cytometric DNA analysis was performed with paraffin-embedded specimens from tumors of 107 patients with EGC. Flow cytometric analysis was performed using a FACScan (Becton Dickinson). In constructing the histogram, 30,000 cells were scanned from each section and results were scored. The S-phase fraction was obtained according to the CellFit cell cycle analysis (Becton Dikinson). Frequencies of aneuploidy in tumors with various clinical and pathologic parameters were compared using the chi-square test. Mean SPF/PI valuse were compared by the student t-test. RESULTS: Diploidy pattern was observed in 80 (75%) cases while aneuploidy was seen in 27(25%) cases. Aneuploidy was more frequently detected in tumors with submucosal involvement (32.7%) and lymph node (+) group (30.8%) than in the mucosal tumor (17.3%) and lymph node (-) group (24.5%), but the differences were not significant. Frequency of aneuploidy was not affected by either the histologic type or morphologic classification. On the other hand, high proliferative activities (SPF/PI) significantly correlated with the submucosal tumor invasion (66.7% vs. 45%; p < 0.05) and lymph node metastasis (28.6% vs. 7.5%; p < 0.05). CONCLUSION: Tumor aggressiveness is not directly related to DNA aneuploidy but proliferative activities are responsible for the aggressive nature of early gastric cancer. The results of this study show that DNA analysis by flow cytometry in considered to be one method of determining the biological activity of gastric cancer cells.

Original languageEnglish
Pages (from-to)137-143
Number of pages7
JournalThe Korean journal of internal medicine
Volume12
Issue number2
DOIs
Publication statusPublished - 1997 Jun

Fingerprint

Stomach Neoplasms
Flow Cytometry
Aneuploidy
DNA
Neoplasms
Lymph Nodes
Paraffin
Chi-Square Distribution
Diploidy
S Phase
Cell Cycle
Students
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Kim, J. M. ; Lee, D. K. ; Kim, Y. K. ; Baik, S. K. ; Lee, C. I. ; Kwon, S. O. ; Jung, S. H. / DNA analysis by flow cytometry in early gastric cancer. In: The Korean journal of internal medicine. 1997 ; Vol. 12, No. 2. pp. 137-143.
@article{475e5718af7a4a0cab66fb4857540208,
title = "DNA analysis by flow cytometry in early gastric cancer.",
abstract = "OBJECTIVES: Flow cytometric analysis of a paraffin-embedded block of tissue provides rapid and accurate means of analyzing the DNA content of a tumor. The aim of this study was to clarify the clinical significance of flow cytometric findings in early gastric cancer(EGC). Thus we conducted this study to investigate whether DNA contents of tumor cells can correlate with known prognostic indices in patients with EGC. METHODS: The flow cytometric DNA analysis was performed with paraffin-embedded specimens from tumors of 107 patients with EGC. Flow cytometric analysis was performed using a FACScan (Becton Dickinson). In constructing the histogram, 30,000 cells were scanned from each section and results were scored. The S-phase fraction was obtained according to the CellFit cell cycle analysis (Becton Dikinson). Frequencies of aneuploidy in tumors with various clinical and pathologic parameters were compared using the chi-square test. Mean SPF/PI valuse were compared by the student t-test. RESULTS: Diploidy pattern was observed in 80 (75{\%}) cases while aneuploidy was seen in 27(25{\%}) cases. Aneuploidy was more frequently detected in tumors with submucosal involvement (32.7{\%}) and lymph node (+) group (30.8{\%}) than in the mucosal tumor (17.3{\%}) and lymph node (-) group (24.5{\%}), but the differences were not significant. Frequency of aneuploidy was not affected by either the histologic type or morphologic classification. On the other hand, high proliferative activities (SPF/PI) significantly correlated with the submucosal tumor invasion (66.7{\%} vs. 45{\%}; p < 0.05) and lymph node metastasis (28.6{\%} vs. 7.5{\%}; p < 0.05). CONCLUSION: Tumor aggressiveness is not directly related to DNA aneuploidy but proliferative activities are responsible for the aggressive nature of early gastric cancer. The results of this study show that DNA analysis by flow cytometry in considered to be one method of determining the biological activity of gastric cancer cells.",
author = "Kim, {J. M.} and Lee, {D. K.} and Kim, {Y. K.} and Baik, {S. K.} and Lee, {C. I.} and Kwon, {S. O.} and Jung, {S. H.}",
year = "1997",
month = "6",
doi = "10.3904/kjim.1997.12.2.137",
language = "English",
volume = "12",
pages = "137--143",
journal = "Korean Journal of Internal Medicine",
issn = "1226-3303",
publisher = "Korean Association of Internal Medicine",
number = "2",

}

DNA analysis by flow cytometry in early gastric cancer. / Kim, J. M.; Lee, D. K.; Kim, Y. K.; Baik, S. K.; Lee, C. I.; Kwon, S. O.; Jung, S. H.

In: The Korean journal of internal medicine, Vol. 12, No. 2, 06.1997, p. 137-143.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA analysis by flow cytometry in early gastric cancer.

AU - Kim, J. M.

AU - Lee, D. K.

AU - Kim, Y. K.

AU - Baik, S. K.

AU - Lee, C. I.

AU - Kwon, S. O.

AU - Jung, S. H.

PY - 1997/6

Y1 - 1997/6

N2 - OBJECTIVES: Flow cytometric analysis of a paraffin-embedded block of tissue provides rapid and accurate means of analyzing the DNA content of a tumor. The aim of this study was to clarify the clinical significance of flow cytometric findings in early gastric cancer(EGC). Thus we conducted this study to investigate whether DNA contents of tumor cells can correlate with known prognostic indices in patients with EGC. METHODS: The flow cytometric DNA analysis was performed with paraffin-embedded specimens from tumors of 107 patients with EGC. Flow cytometric analysis was performed using a FACScan (Becton Dickinson). In constructing the histogram, 30,000 cells were scanned from each section and results were scored. The S-phase fraction was obtained according to the CellFit cell cycle analysis (Becton Dikinson). Frequencies of aneuploidy in tumors with various clinical and pathologic parameters were compared using the chi-square test. Mean SPF/PI valuse were compared by the student t-test. RESULTS: Diploidy pattern was observed in 80 (75%) cases while aneuploidy was seen in 27(25%) cases. Aneuploidy was more frequently detected in tumors with submucosal involvement (32.7%) and lymph node (+) group (30.8%) than in the mucosal tumor (17.3%) and lymph node (-) group (24.5%), but the differences were not significant. Frequency of aneuploidy was not affected by either the histologic type or morphologic classification. On the other hand, high proliferative activities (SPF/PI) significantly correlated with the submucosal tumor invasion (66.7% vs. 45%; p < 0.05) and lymph node metastasis (28.6% vs. 7.5%; p < 0.05). CONCLUSION: Tumor aggressiveness is not directly related to DNA aneuploidy but proliferative activities are responsible for the aggressive nature of early gastric cancer. The results of this study show that DNA analysis by flow cytometry in considered to be one method of determining the biological activity of gastric cancer cells.

AB - OBJECTIVES: Flow cytometric analysis of a paraffin-embedded block of tissue provides rapid and accurate means of analyzing the DNA content of a tumor. The aim of this study was to clarify the clinical significance of flow cytometric findings in early gastric cancer(EGC). Thus we conducted this study to investigate whether DNA contents of tumor cells can correlate with known prognostic indices in patients with EGC. METHODS: The flow cytometric DNA analysis was performed with paraffin-embedded specimens from tumors of 107 patients with EGC. Flow cytometric analysis was performed using a FACScan (Becton Dickinson). In constructing the histogram, 30,000 cells were scanned from each section and results were scored. The S-phase fraction was obtained according to the CellFit cell cycle analysis (Becton Dikinson). Frequencies of aneuploidy in tumors with various clinical and pathologic parameters were compared using the chi-square test. Mean SPF/PI valuse were compared by the student t-test. RESULTS: Diploidy pattern was observed in 80 (75%) cases while aneuploidy was seen in 27(25%) cases. Aneuploidy was more frequently detected in tumors with submucosal involvement (32.7%) and lymph node (+) group (30.8%) than in the mucosal tumor (17.3%) and lymph node (-) group (24.5%), but the differences were not significant. Frequency of aneuploidy was not affected by either the histologic type or morphologic classification. On the other hand, high proliferative activities (SPF/PI) significantly correlated with the submucosal tumor invasion (66.7% vs. 45%; p < 0.05) and lymph node metastasis (28.6% vs. 7.5%; p < 0.05). CONCLUSION: Tumor aggressiveness is not directly related to DNA aneuploidy but proliferative activities are responsible for the aggressive nature of early gastric cancer. The results of this study show that DNA analysis by flow cytometry in considered to be one method of determining the biological activity of gastric cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=0031151496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031151496&partnerID=8YFLogxK

U2 - 10.3904/kjim.1997.12.2.137

DO - 10.3904/kjim.1997.12.2.137

M3 - Article

C2 - 9439148

AN - SCOPUS:0031151496

VL - 12

SP - 137

EP - 143

JO - Korean Journal of Internal Medicine

JF - Korean Journal of Internal Medicine

SN - 1226-3303

IS - 2

ER -