DNA-binding cell-penetrating peptide-based TRAIL over-expression in adipose tissue-derived mesenchymal stem cells inhibits glioma U251MG growth

Jaesik Shin, Soon Koo Baik, Yongdae Yoon, Soonjae Hwang, Joon Hyung Sohn, Minjeong Jo, Woo Seung Kim, Ki Jong Rhee, Kum Whang, Young Woo Eom

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: Genetic manipulation of stem cells using non-viral vectors is still limited due to low transfection efficiency. We investigated whether the DNAbinding cell-permeation peptides (CPP) can enhance the transfection efficiency of non-viral vectors in adipose tissuederived mesenchymal stem cells (ASCs) and whether ASCs over-expressing TRAIL through CPP can inhibit the growth of glioma U251MG cells in vitro and in vivo. Materials and Methods: ASCs were genetically engineered to over-express TRAIL by using CPP, pCMV3-TRAIL and lipid-based transfection reagents (X-tremeGENE). Results: The transfection efficiency of ASCs increased by approximately 7% using CPP; 53.9% of ASCs were transfected and TRAIL expression in ASCs increased by approximately 3 times compared to X-tremeGENE alone. ASCs over-expressing TRAIL using CPP inhibited growth of glioma U251MG cells both in vitro and in the U251MG xenograft model. Conclusion: CPP can be used as an enhancer for genetically manipulating ASCs and tumor treatment.

Original languageEnglish
Pages (from-to)2859-2866
Number of pages8
JournalAnticancer research
Volume41
Issue number6
DOIs
Publication statusPublished - 2021 Jun

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI17C1365), and the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Korean government, the Ministry of Education (NRF-2017R1D1A1A02019212).

Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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