DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii

Ki Chang Keum, Seung Min Yoo, Sang Yup Lee, Kyung Hee Chang, Nae Choon Yoo, Won Min Yoo, June Myung Kim, Jun Yong Choi, Jong Seok Kim, Gene Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.

Original languageEnglish
Pages (from-to)42-50
Number of pages9
JournalMolecular and Cellular Probes
Volume20
Issue number1
DOIs
Publication statusPublished - 2006 Feb 1

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Acinetobacter baumannii
Oligonucleotide Array Sequence Analysis
Pseudomonas aeruginosa
Bacteria
Suppuration
Oligonucleotide Probes
Cross Infection
Ribosomal DNA
Sputum
Cerebrospinal Fluid
Urine
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Keum, Ki Chang ; Yoo, Seung Min ; Lee, Sang Yup ; Chang, Kyung Hee ; Yoo, Nae Choon ; Yoo, Won Min ; Kim, June Myung ; Choi, Jun Yong ; Kim, Jong Seok ; Lee, Gene. / DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii. In: Molecular and Cellular Probes. 2006 ; Vol. 20, No. 1. pp. 42-50.
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DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii. / Keum, Ki Chang; Yoo, Seung Min; Lee, Sang Yup; Chang, Kyung Hee; Yoo, Nae Choon; Yoo, Won Min; Kim, June Myung; Choi, Jun Yong; Kim, Jong Seok; Lee, Gene.

In: Molecular and Cellular Probes, Vol. 20, No. 1, 01.02.2006, p. 42-50.

Research output: Contribution to journalArticle

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