DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication

Seho Cha, Chunghun Lim, Jae Young Lee, Yoon Jae Song, Junsoo Park, Joonho Choe, Taegun Seo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

During latent infection, latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/-) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.

Original languageEnglish
Pages (from-to)934-939
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume394
Issue number4
DOIs
Publication statusPublished - 2010 Apr 16

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Human Herpesvirus 8
HCT116 Cells
DNA
Polynucleotide 5'-Hydroxyl-Kinase
Catalytic DNA
Cell Line
Cells
Protein Kinases
Catalytic Domain
Infection
latency-associated nuclear antigen

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication",
abstract = "During latent infection, latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/-) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.",
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DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication. / Cha, Seho; Lim, Chunghun; Lee, Jae Young; Song, Yoon Jae; Park, Junsoo; Choe, Joonho; Seo, Taegun.

In: Biochemical and Biophysical Research Communications, Vol. 394, No. 4, 16.04.2010, p. 934-939.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication

AU - Cha, Seho

AU - Lim, Chunghun

AU - Lee, Jae Young

AU - Song, Yoon Jae

AU - Park, Junsoo

AU - Choe, Joonho

AU - Seo, Taegun

PY - 2010/4/16

Y1 - 2010/4/16

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AB - During latent infection, latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/-) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.

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