Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent

Kyoung Hwa Lee, Myon Hee Lee, Tae Hoon Lee, Ji Won Han, Yong Jin Park, Joohong Ahnn, Yeon Soo Seo, Hyeon-Sook Koo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To determine the function of Dna2 in a multicellular organism, the Caenorhabditis elegans Dna2 expression was probed and deletion mutant phenotypes were analyzed. Dna2 was localized to the nuclei of C. elegans oocytes and early embryos by immunostaining or green fluorescent protein-tagging. A homozygous dna2 deletion mutant showed a reduced brood size and embryonic lethality, and the phenotypes greatly depended on growth temperature and aggravated in the succeeding generation. The mutant embryos also showed delayed cell divisions, which together with temperature-dependence of the mutant phenotypes supported the well-conserved role of Dna2 in DNA replication.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalMolecules and cells
Volume15
Issue number1
Publication statusPublished - 2003 Feb 1

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Caenorhabditis elegans
Reproduction
Phenotype
Temperature
Embryonic Structures
Green Fluorescent Proteins
DNA Replication
Cell Division
Oocytes
Growth

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, K. H., Lee, M. H., Lee, T. H., Han, J. W., Park, Y. J., Ahnn, J., ... Koo, H-S. (2003). Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent. Molecules and cells, 15(1), 81-86.
Lee, Kyoung Hwa ; Lee, Myon Hee ; Lee, Tae Hoon ; Han, Ji Won ; Park, Yong Jin ; Ahnn, Joohong ; Seo, Yeon Soo ; Koo, Hyeon-Sook. / Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent. In: Molecules and cells. 2003 ; Vol. 15, No. 1. pp. 81-86.
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Lee, KH, Lee, MH, Lee, TH, Han, JW, Park, YJ, Ahnn, J, Seo, YS & Koo, H-S 2003, 'Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent', Molecules and cells, vol. 15, no. 1, pp. 81-86.

Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent. / Lee, Kyoung Hwa; Lee, Myon Hee; Lee, Tae Hoon; Han, Ji Won; Park, Yong Jin; Ahnn, Joohong; Seo, Yeon Soo; Koo, Hyeon-Sook.

In: Molecules and cells, Vol. 15, No. 1, 01.02.2003, p. 81-86.

Research output: Contribution to journalArticle

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AU - Koo, Hyeon-Sook

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Lee KH, Lee MH, Lee TH, Han JW, Park YJ, Ahnn J et al. Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent. Molecules and cells. 2003 Feb 1;15(1):81-86.