Abstract
Abstract: Class I regulatory complex (CRC) located in the 5′‐upstream region of MHC class I gene contains transcriptional enhancing sequences, called Enh A. This Enh A region contains tandem‐arranged κB‐like sites, one of which has a well‐conserved perfect palindromic sequence. The second κB‐like site, juxtaposed to the perfect palindrome, contains an imperfect palindromic sequence. In B‐cell nuclear extracts, we have identified at least four sequence‐specific protein complexes; three shared the repeated κB enhancer as their binding motifs. The perfect palindromic sequence facilitates the binding of a complex termed BI, while the imperfect palindrome provides the binding sites for two other complexes, BII and BIII. The BII and BIII complexes exhibited binding crossreactivity with other κB‐related motifs and recognized both the perfect and imperfect palindromic sequences, whereas the BI complex was specific for the perfect palindromic sequence which is unique to the class I promoters. A DNA segment outside the repeated κB enhancers probably binds the fourth complex, BIV. These complexes, except for the perfect palindrome‐binding complex, differ from those described for the murine class I promoter. The binding characteristics of these factors suggest that the mechanism controlling the class I transcription may be quite complex. 1993 Blackwell Munksgaard
| Original language | English |
|---|---|
| Pages (from-to) | 78-86 |
| Number of pages | 9 |
| Journal | Tissue Antigens |
| Volume | 42 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1993 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Biochemistry
- Genetics
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DNA‐binding proteins for transcription enhancing region of HLA class I gene. / Park, Jean Han; Lee, Han Woong; Fleischhauer, Katharina L.; Kim, Chul Gun; Sheffery, Michael; Yang, Soo Young.
In: Tissue Antigens, Vol. 42, No. 1, 01.01.1993, p. 78-86.Research output: Contribution to journal › Article
TY - JOUR
T1 - DNA‐binding proteins for transcription enhancing region of HLA class I gene
AU - Park, Jean Han
AU - Lee, Han Woong
AU - Fleischhauer, Katharina L.
AU - Kim, Chul Gun
AU - Sheffery, Michael
AU - Yang, Soo Young
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Abstract: Class I regulatory complex (CRC) located in the 5′‐upstream region of MHC class I gene contains transcriptional enhancing sequences, called Enh A. This Enh A region contains tandem‐arranged κB‐like sites, one of which has a well‐conserved perfect palindromic sequence. The second κB‐like site, juxtaposed to the perfect palindrome, contains an imperfect palindromic sequence. In B‐cell nuclear extracts, we have identified at least four sequence‐specific protein complexes; three shared the repeated κB enhancer as their binding motifs. The perfect palindromic sequence facilitates the binding of a complex termed BI, while the imperfect palindrome provides the binding sites for two other complexes, BII and BIII. The BII and BIII complexes exhibited binding crossreactivity with other κB‐related motifs and recognized both the perfect and imperfect palindromic sequences, whereas the BI complex was specific for the perfect palindromic sequence which is unique to the class I promoters. A DNA segment outside the repeated κB enhancers probably binds the fourth complex, BIV. These complexes, except for the perfect palindrome‐binding complex, differ from those described for the murine class I promoter. The binding characteristics of these factors suggest that the mechanism controlling the class I transcription may be quite complex. 1993 Blackwell Munksgaard
AB - Abstract: Class I regulatory complex (CRC) located in the 5′‐upstream region of MHC class I gene contains transcriptional enhancing sequences, called Enh A. This Enh A region contains tandem‐arranged κB‐like sites, one of which has a well‐conserved perfect palindromic sequence. The second κB‐like site, juxtaposed to the perfect palindrome, contains an imperfect palindromic sequence. In B‐cell nuclear extracts, we have identified at least four sequence‐specific protein complexes; three shared the repeated κB enhancer as their binding motifs. The perfect palindromic sequence facilitates the binding of a complex termed BI, while the imperfect palindrome provides the binding sites for two other complexes, BII and BIII. The BII and BIII complexes exhibited binding crossreactivity with other κB‐related motifs and recognized both the perfect and imperfect palindromic sequences, whereas the BI complex was specific for the perfect palindromic sequence which is unique to the class I promoters. A DNA segment outside the repeated κB enhancers probably binds the fourth complex, BIV. These complexes, except for the perfect palindrome‐binding complex, differ from those described for the murine class I promoter. The binding characteristics of these factors suggest that the mechanism controlling the class I transcription may be quite complex. 1993 Blackwell Munksgaard
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UR - http://www.scopus.com/inward/citedby.url?scp=0027377428&partnerID=8YFLogxK
U2 - 10.1111/j.1399-0039.1993.tb02171.x
DO - 10.1111/j.1399-0039.1993.tb02171.x
M3 - Article
C2 - 8266321
AN - SCOPUS:0027377428
VL - 42
SP - 78
EP - 86
JO - HLA
JF - HLA
SN - 2059-2302
IS - 1
ER -