DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection

Pratik Shah, Suk Won Choi, Ho Jin Kim, Seok Keun Cho, Peter Waaben Thulstrup, Morten Jannik Bjerrum, Yong Joo Bhang, Jong Cheol Ahn, Seong Wook Yang

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16 Citations (Scopus)

Abstract

In recent years microRNAs (miRNAs) have been established as important biomarkers in a variety of diseases including cancer, diabetes, cardiovascular disease, aging, Alzheimer's disease, asthma, autoimmune disease and liver diseases. As a consequence, a variety of monitoring methods for miRNAs have been developed, including a fast and simple method for miRNA detection by exploitation of the unique photoluminescence of DNA-templated silver nanoclusters (DNA/AgNCs). To increase the versatility of the AgNC-based method, we have adopted DNA/RNA chimera templates for AgNC-based probes, allowing response from several human miRNAs which are hardly detectable with DNA-based probes. Here, we demonstrate in detail the power of DNA/RNA chimera/AgNC probes in detecting two human miRNAs, let-7a and miR-200c. The DNA/RNA chimera-based probes are highly efficient to determine the level of miRNAs in several human cell lines.

Original languageEnglish
Pages (from-to)3422-3430
Number of pages9
JournalAnalyst
Volume140
Issue number10
DOIs
Publication statusPublished - 2015 May 21

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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    Shah, P., Choi, S. W., Kim, H. J., Cho, S. K., Thulstrup, P. W., Bjerrum, M. J., Bhang, Y. J., Ahn, J. C., & Yang, S. W. (2015). DNA/RNA chimera templates improve the emission intensity and target the accessibility of silver nanocluster-based sensors for human microRNA detection. Analyst, 140(10), 3422-3430. https://doi.org/10.1039/c5an00093a