Does the intestinal microbial community of Korean Crohn's disease patients differ from that of western patients?

Chang Soo Eun, Min Jung Kwak, Dong Soo Han, A. Reum Lee, Dong Il Park, Suk Kyun Yang, Yong Seok Kim, Jihyun F. Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Intestinal microbiota play an important role in maintaining the homeostasis of the host immune system. To analyze the alteration of the intestinal microbial community structure in Korean Crohn's disease (CD) patients, we performed a comparative metagenomic analysis between healthy people and CD patients using fecal samples and mucosal tissues of ileocecal valve. Methods: 16S rRNA genes from fecal samples or mucosal tissues of 35 CD patients and 15 healthy controls (HC) were amplified using a universal primer set and sequenced with GS FLX Titanium. The microbial composition and diversity of each sample were analyzed with the mothur pipeline, and the association between microbial community and clinical characteristics of the patients were investigated. Results: The contribution of bacterial groups to the intestinal microbial composition differed between CD and HC, especially in fecal samples. Global structure and individual bacterial abundance of intestinal microbial community were different between feces and ileocecal tissues in HC. In CD patients with active stage, relative abundances of Gammaproteobacteria and Fusobacteria were higher in both fecal and mucosal tissue samples. Moreover, the intestinal microbial community structure was altered by anti-tumor necrosis factor (anti-TNF) treatment. Conclusions: Our 16S rRNA sequence data demonstrate intestinal dysbiosis at the community level in Korean CD patients, which is similar to alterations of the intestinal microbial community seen in the western counterparts. Clinical disease activity and anti-TNF treatment might affect the intestinal microbial community structure in CD patients.

Original languageEnglish
Article number28
JournalBMC Gastroenterology
Volume16
Issue number1
DOIs
Publication statusPublished - 2016 Feb 29

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Crohn Disease
Mucous Membrane
Fusobacteria
Tumor Necrosis Factor-alpha
Dysbiosis
Ileocecal Valve
Gammaproteobacteria
Bacterial Structures
Metagenomics
Titanium
rRNA Genes
Feces
Immune System
Homeostasis
Therapeutics

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Eun, Chang Soo ; Kwak, Min Jung ; Han, Dong Soo ; Lee, A. Reum ; Park, Dong Il ; Yang, Suk Kyun ; Kim, Yong Seok ; Kim, Jihyun F. / Does the intestinal microbial community of Korean Crohn's disease patients differ from that of western patients?. In: BMC Gastroenterology. 2016 ; Vol. 16, No. 1.
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abstract = "Background: Intestinal microbiota play an important role in maintaining the homeostasis of the host immune system. To analyze the alteration of the intestinal microbial community structure in Korean Crohn's disease (CD) patients, we performed a comparative metagenomic analysis between healthy people and CD patients using fecal samples and mucosal tissues of ileocecal valve. Methods: 16S rRNA genes from fecal samples or mucosal tissues of 35 CD patients and 15 healthy controls (HC) were amplified using a universal primer set and sequenced with GS FLX Titanium. The microbial composition and diversity of each sample were analyzed with the mothur pipeline, and the association between microbial community and clinical characteristics of the patients were investigated. Results: The contribution of bacterial groups to the intestinal microbial composition differed between CD and HC, especially in fecal samples. Global structure and individual bacterial abundance of intestinal microbial community were different between feces and ileocecal tissues in HC. In CD patients with active stage, relative abundances of Gammaproteobacteria and Fusobacteria were higher in both fecal and mucosal tissue samples. Moreover, the intestinal microbial community structure was altered by anti-tumor necrosis factor (anti-TNF) treatment. Conclusions: Our 16S rRNA sequence data demonstrate intestinal dysbiosis at the community level in Korean CD patients, which is similar to alterations of the intestinal microbial community seen in the western counterparts. Clinical disease activity and anti-TNF treatment might affect the intestinal microbial community structure in CD patients.",
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Does the intestinal microbial community of Korean Crohn's disease patients differ from that of western patients? / Eun, Chang Soo; Kwak, Min Jung; Han, Dong Soo; Lee, A. Reum; Park, Dong Il; Yang, Suk Kyun; Kim, Yong Seok; Kim, Jihyun F.

In: BMC Gastroenterology, Vol. 16, No. 1, 28, 29.02.2016.

Research output: Contribution to journalArticle

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T1 - Does the intestinal microbial community of Korean Crohn's disease patients differ from that of western patients?

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AU - Kwak, Min Jung

AU - Han, Dong Soo

AU - Lee, A. Reum

AU - Park, Dong Il

AU - Yang, Suk Kyun

AU - Kim, Yong Seok

AU - Kim, Jihyun F.

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AB - Background: Intestinal microbiota play an important role in maintaining the homeostasis of the host immune system. To analyze the alteration of the intestinal microbial community structure in Korean Crohn's disease (CD) patients, we performed a comparative metagenomic analysis between healthy people and CD patients using fecal samples and mucosal tissues of ileocecal valve. Methods: 16S rRNA genes from fecal samples or mucosal tissues of 35 CD patients and 15 healthy controls (HC) were amplified using a universal primer set and sequenced with GS FLX Titanium. The microbial composition and diversity of each sample were analyzed with the mothur pipeline, and the association between microbial community and clinical characteristics of the patients were investigated. Results: The contribution of bacterial groups to the intestinal microbial composition differed between CD and HC, especially in fecal samples. Global structure and individual bacterial abundance of intestinal microbial community were different between feces and ileocecal tissues in HC. In CD patients with active stage, relative abundances of Gammaproteobacteria and Fusobacteria were higher in both fecal and mucosal tissue samples. Moreover, the intestinal microbial community structure was altered by anti-tumor necrosis factor (anti-TNF) treatment. Conclusions: Our 16S rRNA sequence data demonstrate intestinal dysbiosis at the community level in Korean CD patients, which is similar to alterations of the intestinal microbial community seen in the western counterparts. Clinical disease activity and anti-TNF treatment might affect the intestinal microbial community structure in CD patients.

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