Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model

Yoon Hee Cho, Dae Sung Kim, Peter Geon Kim, Yong Sup Hwang, Myung Soo Cho, Shin Yong Moon, Dong Wook Kim, Jin Woo Chang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells (∼28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.

Original languageEnglish
Pages (from-to)6-12
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume341
Issue number1
DOIs
Publication statusPublished - 2006 Mar 3

Bibliographical note

Funding Information:
This research was supported by a Grant (03-PJ1-PG1-CH07-0004) from the Korea Health 21 R&D Project, Ministry of Health & Welfare and Grants (codes: SC1020, SC2140, and SC2160) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are grateful to Dr. Kwang-Soo Kim at Harvard Medical School/Mclean Hospital for the gift of N2 cells.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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