Dose-related cytoprotective effect of alpha-lipoic acid on hydrogen peroxide-induced oxidative stress to pancreatic beta cells.

byungwan lee, Soo Jin Kwon, Hee Young Chae, Jun Goo Kang, Chul Sik Kim, Seong Jin Lee, Hyung Joon Yoo, Jae Hyeon Kim, Kyong Soo Park, Sung Hee Ihm

Research output: Contribution to journalArticle

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Abstract

alpha-Lipoic acid (alpha-LA), an antioxidant used for diabetic polyneuropathy, was reported to induce AMP-activated protein kinase activation and reductions in insulin secretion in pancreatic beta-cells at high concentrations (> or = 500 micromol/l). This study investigated whether alpha-LA has a protective role under oxidative stress in beta-cells and its effect is dose-related. In INS-1 cells treated with alpha-LA (150-1200 micromol/l) for 24 h, alpha-LA itself (> or = 300 micromol/l) induced apoptotic death dose-dependently. However, pre-treatment with 150 and 300 micromol/l alpha-LA reduced the hydrogen peroxide-induced apoptosis in INS-1 cells and isolated islets. alpha-LA alleviated hydrogen peroxide-induced reactive oxygen species production, mitochondrial membrane depolarization and c-JNK activation in beta-cells. alpha-LA induced phosphoinositide 3-kinase-dependent Akt phosphorylation in INS-1 cells. While alpha-LA is harmful to beta-cells at high concentrations in vitro, it has potential cytoprotective effects on beta-cells under oxidative stress as in diabetes by its antioxidant properties and possibly by Akt phosphorylation at clinically relevant concentrations.

Original languageEnglish
Pages (from-to)68-77
Number of pages10
JournalFree radical research
Volume43
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

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Thioctic Acid
Oxidative stress
Insulin-Secreting Cells
Hydrogen Peroxide
Oxidative Stress
Phosphorylation
Antioxidants
Chemical activation
AMP-Activated Protein Kinases
1-Phosphatidylinositol 4-Kinase
Diabetic Neuropathies
Depolarization
Mitochondrial Membranes
Medical problems
Phosphatidylinositols
Islets of Langerhans
Reactive Oxygen Species
Phosphotransferases
Insulin
Apoptosis

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

lee, byungwan ; Kwon, Soo Jin ; Chae, Hee Young ; Kang, Jun Goo ; Kim, Chul Sik ; Lee, Seong Jin ; Yoo, Hyung Joon ; Kim, Jae Hyeon ; Park, Kyong Soo ; Ihm, Sung Hee. / Dose-related cytoprotective effect of alpha-lipoic acid on hydrogen peroxide-induced oxidative stress to pancreatic beta cells. In: Free radical research. 2009 ; Vol. 43, No. 1. pp. 68-77.
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Dose-related cytoprotective effect of alpha-lipoic acid on hydrogen peroxide-induced oxidative stress to pancreatic beta cells. / lee, byungwan; Kwon, Soo Jin; Chae, Hee Young; Kang, Jun Goo; Kim, Chul Sik; Lee, Seong Jin; Yoo, Hyung Joon; Kim, Jae Hyeon; Park, Kyong Soo; Ihm, Sung Hee.

In: Free radical research, Vol. 43, No. 1, 01.01.2009, p. 68-77.

Research output: Contribution to journalArticle

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