Abstract
Important factors involved in odontogenesis in mouse dental papillae disappear between the pre-and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae.
| Original language | English |
|---|---|
| Pages (from-to) | 679-683 |
| Number of pages | 5 |
| Journal | Journal of Dental Research |
| Volume | 89 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2010 Jul |
Bibliographical note
Funding Information:This work was supported by the Japanese Ministry of Education, Culture, Sports, Science and Technology (Grant-in-Aid No. 17591926).
All Science Journal Classification (ASJC) codes
- Dentistry(all)