Down regulation of extramacrochaetae mRNA by a Drosophila neural RNA binding protein Rbp9 which is homologous to human Hu proteins

Soon Jung Park, Eun Sung Yang, Jeongsil Kim-Ha, Young Joon Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Rbp9 is an RNA binding protein expressed mainly in the central nervous system of adult Drosophila melanogaster. Rbp9 shares a high degree of sequence similarity with human neural proteins referred to as Hu antigens. Hu antigens bind to U-rich mRNA destabilizing elements with a high affinity and, thus, have been implicated as regulators of mRNA stability. Using in vitro RNA binding assays, we found that Rbp9 binds strongly to poly U sequences. We then employed a Selex system to identify a consensus Rbp9 binding site (UUUXUUUU). Information obtained from the Selex results allowed the detection of two repeats of the Rbp9 consensus binding sequence in the 3' untranslated region of extramacrochaetae mRNA. UV crosslinking experiments demonstrated that Rbp9 interacts specifically with emc mRNA. The requirement of Rbp9 protein in the down regulation of emc mRNA was confirmed by northern (RNA) analysis, which revealed that the level of emc mRNA increased 10-fold in rbp9 mutant flies. Taken together with the in vitro RNA binding results, the genetic evidence obtained strongly supports the hypothesis that Rbp9 functions as a regulator of RNA stability.

Original languageEnglish
Pages (from-to)2989-2994
Number of pages6
JournalNucleic acids research
Volume26
Issue number12
DOIs
Publication statusPublished - 1998 Jun 15

Fingerprint

RNA-Binding Proteins
Drosophila
Down-Regulation
ELAV Proteins
Messenger RNA
RNA Stability
RNA
Proteins
Poly U
Consensus Sequence
3' Untranslated Regions
Drosophila melanogaster
Diptera
Central Nervous System
Binding Sites

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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abstract = "Rbp9 is an RNA binding protein expressed mainly in the central nervous system of adult Drosophila melanogaster. Rbp9 shares a high degree of sequence similarity with human neural proteins referred to as Hu antigens. Hu antigens bind to U-rich mRNA destabilizing elements with a high affinity and, thus, have been implicated as regulators of mRNA stability. Using in vitro RNA binding assays, we found that Rbp9 binds strongly to poly U sequences. We then employed a Selex system to identify a consensus Rbp9 binding site (UUUXUUUU). Information obtained from the Selex results allowed the detection of two repeats of the Rbp9 consensus binding sequence in the 3' untranslated region of extramacrochaetae mRNA. UV crosslinking experiments demonstrated that Rbp9 interacts specifically with emc mRNA. The requirement of Rbp9 protein in the down regulation of emc mRNA was confirmed by northern (RNA) analysis, which revealed that the level of emc mRNA increased 10-fold in rbp9 mutant flies. Taken together with the in vitro RNA binding results, the genetic evidence obtained strongly supports the hypothesis that Rbp9 functions as a regulator of RNA stability.",
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Down regulation of extramacrochaetae mRNA by a Drosophila neural RNA binding protein Rbp9 which is homologous to human Hu proteins. / Park, Soon Jung; Yang, Eun Sung; Kim-Ha, Jeongsil; Kim, Young Joon.

In: Nucleic acids research, Vol. 26, No. 12, 15.06.1998, p. 2989-2994.

Research output: Contribution to journalArticle

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