Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

Hae Jin Song, Joongkyu Park, Su Ryeon Seo, Jongsun Kim, Seung R. Paik, Kwang Chul Chung

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7 Citations (Scopus)

Abstract

Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume376
Issue number3
DOIs
Publication statusPublished - 2008 Nov 21

Bibliographical note

Funding Information:
We thank F. Henrique-Silvia, R.M. Evans, and J.P. Vanden-Heuvel for providing plasmids. This work was supported by grants from the Brain Research Center of the 21st Century Frontier Research Program Technology (M103KV010011-06K2201-01110 to K.C.C.) and from the Korea Science and Engineering Foundation (KOSEF) through National Research Laboratory Program (R04-2007-000-20014-0 to K.C.C.) funded by the Ministry of Science, Republic of Korea. This work was also partly supported by KOSEF grants (R11-2007-040-01005-0 and R01-2007-000-20089-0 to K.C.C.), a grant from the Korea Health 21 R&D Project (A080551 and A060440 to K.C.C.), Ministry of Health & Welfare, and by the Korea Research Foundation Grant (KRF-2004-005-E0017 to K.C.C.).

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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