Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

Hae Jin Song, Joongkyu Park, Su Ryeon Seo, Jongsun Kim, Seung R. Paik, Kwang Chul Chung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume376
Issue number3
DOIs
Publication statusPublished - 2008 Nov 21

Fingerprint

Peroxisome Proliferator-Activated Receptors
HEK293 Cells
Chromosomes
Assays
Proteins
Fungal Proteins
Chromosomes, Human, Pair 21
Cytoplasmic and Nuclear Receptors
Gene Library
Down Syndrome
Brain
Solubility
Genes
Ligands
Down Syndrome Critical Region
Two-Hybrid System Techniques
Immunoprecipitation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

@article{ba121c64f079486da99976889aa429a8,
title = "Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells",
abstract = "Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.",
author = "Song, {Hae Jin} and Joongkyu Park and Seo, {Su Ryeon} and Jongsun Kim and Paik, {Seung R.} and Chung, {Kwang Chul}",
year = "2008",
month = "11",
day = "21",
doi = "10.1016/j.bbrc.2008.09.017",
language = "English",
volume = "376",
pages = "478--482",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells. / Song, Hae Jin; Park, Joongkyu; Seo, Su Ryeon; Kim, Jongsun; Paik, Seung R.; Chung, Kwang Chul.

In: Biochemical and Biophysical Research Communications, Vol. 376, No. 3, 21.11.2008, p. 478-482.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor β in HEK293 cells

AU - Song, Hae Jin

AU - Park, Joongkyu

AU - Seo, Su Ryeon

AU - Kim, Jongsun

AU - Paik, Seung R.

AU - Chung, Kwang Chul

PY - 2008/11/21

Y1 - 2008/11/21

N2 - Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

AB - Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor β, (PPARβ). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARβ in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARβ. Furthermore, PPARβ also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

UR - http://www.scopus.com/inward/record.url?scp=53149147376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53149147376&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.09.017

DO - 10.1016/j.bbrc.2008.09.017

M3 - Article

VL - 376

SP - 478

EP - 482

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -