Drosophila extracellular signal-regulated kinase involves the insulin-mediated proliferation of schneider cells

Hyung Bae Kwon, Sun Hong Kim, Sung Eun Kim, In Hwan Jang, Yongho Ahn, Won Jae Lee, Kang Yell Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Drosophila insulin pathway is involved in the control of the proliferation and size of the cell. The stimulation of Schneider cells with human insulin has been observed to activate Drosophila extracellular signal regulated kinase (DERK). However, the role of DERK in the regulation of proliferation is unknown. In this study, we have identified a role of DERK in the proliferation of Drosophila Schneider cells. The inhibition of DERK activity by the overexpression of DMKP-3, an ERK-specific mitogen-activated protein kinase (MAPK) phosphatase, inhibited G1 to S phase cell cycle progression as well as bromodeoxyuridine (BrdU) incorporation, which were previously increased by human insulin. However, DMKP-3 overexpression did not significantly reduce cell size that was also enlarged by insulin treatment, which suggests the specificity of the ERK pathway in proliferation but not for cell size. G1 to S phase cell cycle progression and BrdU incorporation were also reduced by catalytically inactive DMKP-3 mutant, and they may be acquired by the trapping of DERK into cytosol. The depletion of DERK or DMKP-3 by inhibitory double-stranded RNA decreased and increased BrdU incorporation, respectively. Thus, we propose that DERK is involved in the proliferation of Schneider cells via the insulin pathway.

Original languageEnglish
Pages (from-to)14853-14858
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number17
DOIs
Publication statusPublished - 2002 Apr 26

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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