Drosophila Med6 is required for elevated expression of a large but distinct set of developmentally regulated genes

Soo Gim Byung Soo Gim, Mo Park Jin Mo Park, Ho Yoon Jeong Ho Yoon, C. Kang, Y. J. Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mediator is the evolutionarily conserved coactivator required for the integration and recruitment of diverse regulatory signals to basal transcription machinery. To elucidate the functions of metazoan Mediator, we isolated Drosophila melanogaster Med6 mutants. dMed6 is essential for viability and/or proliferation of most cells. dMed6 mutants failed to pupate and died in the third larval instar with severe proliferation defects in imaginal discs and other larval mitotic cells. cDNA microarray, quantitative reverse transcription-PCR, and in situ expression analyses of developmentally regulated genes in dMed6 mutants showed that transcriptional activation of many, but not all, genes was affected. Among the genes found to be affected were some that play a role in cell proliferation and metabolism. Therefore, dMed6 is required in most cells for transcriptional regulation of many genes important for diverse aspects of Drosophila development.

Original languageEnglish
Pages (from-to)5242-5255
Number of pages14
JournalMolecular and Cellular Biology
Volume21
Issue number15
DOIs
Publication statusPublished - 2001 Jul 28

Fingerprint

Drosophila
Genes
Cell Proliferation
Imaginal Discs
Oligonucleotide Array Sequence Analysis
Drosophila melanogaster
Transcriptional Activation
Reverse Transcription
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Byung Soo Gim, Soo Gim ; Jin Mo Park, Mo Park ; Jeong Ho Yoon, Ho Yoon ; Kang, C. ; Kim, Y. J. / Drosophila Med6 is required for elevated expression of a large but distinct set of developmentally regulated genes. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 15. pp. 5242-5255.
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Drosophila Med6 is required for elevated expression of a large but distinct set of developmentally regulated genes. / Byung Soo Gim, Soo Gim; Jin Mo Park, Mo Park; Jeong Ho Yoon, Ho Yoon; Kang, C.; Kim, Y. J.

In: Molecular and Cellular Biology, Vol. 21, No. 15, 28.07.2001, p. 5242-5255.

Research output: Contribution to journalArticle

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