In animal circadian clock machinery, the phosphorylation program of PERIOD (PER) leads to the spatio-temporal regulation of diverse PER functions, which are crucial for the maintenance of ∼24-hr circadian rhythmicity. The peptidyl-prolyl isomerase PIN1 modulates the diverse functions of its substrates by inducing conformational changes upon recognizing specific phosphorylated residues. Here, we show that overexpression of Drosophila pin1, dodo (dod), lengthens the locomotor behavioral period. Using Drosophila S2 cells, we demonstrate that Dod associates preferentially with phosphorylated species of PER, which delays the phosphorylation-dependent degradation of PER. Consistent with this, PER protein levels are higher in flies overexpressing dod. Taken together, we suggest that Dod plays a role in the maintenance of circadian period by regulating PER metabolism.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2015 Jun 14|
Bibliographical noteFunding Information:
We thank the members of the EY Kim laboratory for critically reading this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant ( NRF-2014R1A2A1A11051765 , NRF-2012R1A5A2048183 ) funded by the Korean government and a 2011 grant to E.Y.K. from the Department of Medical Sciences funded by the graduate school of Ajou University.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology