DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

Jongjin Park, Yeonsung Son, Na Geum Lee, Kyungmin Lee, Dong Gwang Lee, Jinhoi Song, Jaemin Lee, Seokho Kim, Min Ji Cho, Ju Hong Jang, Jangwook Lee, Jong Gil Park, Yeon Gu Kim, Jang Seong Kim, Jungwoon Lee, Yee Sook Cho, Young Jun Park, Baek Soo Han, Kwang Hee Bae, Seungmin HanByunghoon Kang, Seungjoo Haam, Sang Hyun Lee, Sang Chul Lee, Jeong Ki Min

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

Original languageEnglish
Pages (from-to)115-127
Number of pages13
JournalStem Cell Reports
Issue number1
Publication statusPublished - 2018 Jul 10

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Research Institute of Bioscience and Biotechnology and by grants from the National Research Foundation of Korea , which was funded by the Ministry of Science, Information & Communication Technology and Future Planning ( NRF-2012M3A9C6050331 , NRF-2015M3A9C6030284 , NRF-2015M3A9D7029882 , NRF-2013M3A9B6046566 , and NRF-2012M3A9C7050101 ).

Publisher Copyright:
© 2018 The Authors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology


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