DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

Jongjin Park, Yeonsung Son, Na Geum Lee, Kyungmin Lee, Dong Gwang Lee, Jinhoi Song, Jaemin Lee, Seokho Kim, Min Ji Cho, Ju Hong Jang, Jangwook Lee, Jong Gil Park, Yeon Gu Kim, Jang Seong Kim, Jungwoon Lee, Yee Sook Cho, Young Jun Park, Baek Soo Han, Kwang Hee Bae, Seungmin Han & 5 others Byunghoon Kang, Seungjoo Haam, Sang Hyun Lee, Sang Chul Lee, Jeong Ki Min

Research output: Contribution to journalArticle

Abstract

Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

Original languageEnglish
Pages (from-to)115-127
Number of pages13
JournalStem Cell Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2018 Jul 10

Fingerprint

Desmoglein 2
Forensic Anthropology
Pluripotent Stem Cells
Stem cells
Catenins
Gastropoda
Regenerative Medicine
Epithelial-Mesenchymal Transition
Teratoma
Population
Down-Regulation
Monoclonal Antibodies
Maintenance
Derivatives
Safety
Antigens

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Park, Jongjin ; Son, Yeonsung ; Lee, Na Geum ; Lee, Kyungmin ; Lee, Dong Gwang ; Song, Jinhoi ; Lee, Jaemin ; Kim, Seokho ; Cho, Min Ji ; Jang, Ju Hong ; Lee, Jangwook ; Park, Jong Gil ; Kim, Yeon Gu ; Kim, Jang Seong ; Lee, Jungwoon ; Cho, Yee Sook ; Park, Young Jun ; Han, Baek Soo ; Bae, Kwang Hee ; Han, Seungmin ; Kang, Byunghoon ; Haam, Seungjoo ; Lee, Sang Hyun ; Lee, Sang Chul ; Min, Jeong Ki. / DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells. In: Stem Cell Reports. 2018 ; Vol. 11, No. 1. pp. 115-127.
@article{425d3d5c784a4b249c6b14bccb96afc8,
title = "DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells",
abstract = "Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.",
author = "Jongjin Park and Yeonsung Son and Lee, {Na Geum} and Kyungmin Lee and Lee, {Dong Gwang} and Jinhoi Song and Jaemin Lee and Seokho Kim and Cho, {Min Ji} and Jang, {Ju Hong} and Jangwook Lee and Park, {Jong Gil} and Kim, {Yeon Gu} and Kim, {Jang Seong} and Jungwoon Lee and Cho, {Yee Sook} and Park, {Young Jun} and Han, {Baek Soo} and Bae, {Kwang Hee} and Seungmin Han and Byunghoon Kang and Seungjoo Haam and Lee, {Sang Hyun} and Lee, {Sang Chul} and Min, {Jeong Ki}",
year = "2018",
month = "7",
day = "10",
doi = "10.1016/j.stemcr.2018.05.009",
language = "English",
volume = "11",
pages = "115--127",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "1",

}

Park, J, Son, Y, Lee, NG, Lee, K, Lee, DG, Song, J, Lee, J, Kim, S, Cho, MJ, Jang, JH, Lee, J, Park, JG, Kim, YG, Kim, JS, Lee, J, Cho, YS, Park, YJ, Han, BS, Bae, KH, Han, S, Kang, B, Haam, S, Lee, SH, Lee, SC & Min, JK 2018, 'DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells', Stem Cell Reports, vol. 11, no. 1, pp. 115-127. https://doi.org/10.1016/j.stemcr.2018.05.009

DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells. / Park, Jongjin; Son, Yeonsung; Lee, Na Geum; Lee, Kyungmin; Lee, Dong Gwang; Song, Jinhoi; Lee, Jaemin; Kim, Seokho; Cho, Min Ji; Jang, Ju Hong; Lee, Jangwook; Park, Jong Gil; Kim, Yeon Gu; Kim, Jang Seong; Lee, Jungwoon; Cho, Yee Sook; Park, Young Jun; Han, Baek Soo; Bae, Kwang Hee; Han, Seungmin; Kang, Byunghoon; Haam, Seungjoo; Lee, Sang Hyun; Lee, Sang Chul; Min, Jeong Ki.

In: Stem Cell Reports, Vol. 11, No. 1, 10.07.2018, p. 115-127.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

AU - Park, Jongjin

AU - Son, Yeonsung

AU - Lee, Na Geum

AU - Lee, Kyungmin

AU - Lee, Dong Gwang

AU - Song, Jinhoi

AU - Lee, Jaemin

AU - Kim, Seokho

AU - Cho, Min Ji

AU - Jang, Ju Hong

AU - Lee, Jangwook

AU - Park, Jong Gil

AU - Kim, Yeon Gu

AU - Kim, Jang Seong

AU - Lee, Jungwoon

AU - Cho, Yee Sook

AU - Park, Young Jun

AU - Han, Baek Soo

AU - Bae, Kwang Hee

AU - Han, Seungmin

AU - Kang, Byunghoon

AU - Haam, Seungjoo

AU - Lee, Sang Hyun

AU - Lee, Sang Chul

AU - Min, Jeong Ki

PY - 2018/7/10

Y1 - 2018/7/10

N2 - Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

AB - Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

UR - http://www.scopus.com/inward/record.url?scp=85048174941&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048174941&partnerID=8YFLogxK

U2 - 10.1016/j.stemcr.2018.05.009

DO - 10.1016/j.stemcr.2018.05.009

M3 - Article

VL - 11

SP - 115

EP - 127

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 1

ER -