DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

Jongjin Park; Yeonsung Son; Na Geum Lee; Kyungmin Lee; Dong Gwang Lee; Jinhoi Song; Jaemin Lee; Seokho Kim; Min Ji Cho; Ju Hong Jang; Jangwook Lee; Jong Gil Park; Yeon Gu Kim; Jang Seong Kim; Jungwoon Lee; Yee Sook Cho; Young Jun Park; Baek Soo Han; Kwang Hee Bae; Seungmin Han; Byunghoon Kang; Seungjoo Haam; Sang Hyun Lee; Sang Chul Lee; Jeong Ki Min

doi:10.1016/j.stemcr.2018.05.009

DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

Jongjin Park, Yeonsung Son, Na Geum Lee, Kyungmin Lee, Dong Gwang Lee, Jinhoi Song, Jaemin Lee, Seokho Kim, Min Ji Cho, Ju Hong Jang, Jangwook Lee, Jong Gil Park, Yeon Gu Kim, Jang Seong Kim, Jungwoon Lee, Yee Sook Cho, Young Jun Park, Baek Soo Han, Kwang Hee Bae, Seungmin HanByunghoon Kang, Seungjoo Haam, Sang Hyun Lee, Sang Chul Lee, Jeong Ki Min

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

Original language	English
Pages (from-to)	115-127
Number of pages	13
Journal	Stem Cell Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2018.05.009
Publication status	Published - 2018 Jul 10

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Research Institute of Bioscience and Biotechnology and by grants from the National Research Foundation of Korea , which was funded by the Ministry of Science, Information & Communication Technology and Future Planning ( NRF-2012M3A9C6050331 , NRF-2015M3A9C6030284 , NRF-2015M3A9D7029882 , NRF-2013M3A9B6046566 , and NRF-2012M3A9C7050101 ).

Publisher Copyright:
© 2018 The Authors

All Science Journal Classification (ASJC) codes

Biochemistry
Genetics
Developmental Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.stemcr.2018.05.009

Cite this

Park, J., Son, Y., Lee, N. G., Lee, K., Lee, D. G., Song, J., Lee, J., Kim, S., Cho, M. J., Jang, J. H., Lee, J., Park, J. G., Kim, Y. G., Kim, J. S., Lee, J., Cho, Y. S., Park, Y. J., Han, B. S., Bae, K. H., ... Min, J. K. (2018). DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells. Stem Cell Reports, 11(1), 115-127. https://doi.org/10.1016/j.stemcr.2018.05.009

@article{425d3d5c784a4b249c6b14bccb96afc8,

title = "DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells",

abstract = "Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.",

author = "Jongjin Park and Yeonsung Son and Lee, {Na Geum} and Kyungmin Lee and Lee, {Dong Gwang} and Jinhoi Song and Jaemin Lee and Seokho Kim and Cho, {Min Ji} and Jang, {Ju Hong} and Jangwook Lee and Park, {Jong Gil} and Kim, {Yeon Gu} and Kim, {Jang Seong} and Jungwoon Lee and Cho, {Yee Sook} and Park, {Young Jun} and Han, {Baek Soo} and Bae, {Kwang Hee} and Seungmin Han and Byunghoon Kang and Seungjoo Haam and Lee, {Sang Hyun} and Lee, {Sang Chul} and Min, {Jeong Ki}",

note = "Funding Information: This study was supported by a grant from the Korea Research Institute of Bioscience and Biotechnology and by grants from the National Research Foundation of Korea , which was funded by the Ministry of Science, Information & Communication Technology and Future Planning ( NRF-2012M3A9C6050331 , NRF-2015M3A9C6030284 , NRF-2015M3A9D7029882 , NRF-2013M3A9B6046566 , and NRF-2012M3A9C7050101 ). Publisher Copyright: {\textcopyright} 2018 The Authors",

year = "2018",

month = jul,

day = "10",

doi = "10.1016/j.stemcr.2018.05.009",

language = "English",

volume = "11",

pages = "115--127",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "1",

}

Park, J, Son, Y, Lee, NG, Lee, K, Lee, DG, Song, J, Lee, J, Kim, S, Cho, MJ, Jang, JH, Lee, J, Park, JG, Kim, YG, Kim, JS, Lee, J, Cho, YS, Park, YJ, Han, BS, Bae, KH, Han, S, Kang, B, Haam, S, Lee, SH, Lee, SC & Min, JK 2018, 'DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells', Stem Cell Reports, vol. 11, no. 1, pp. 115-127. https://doi.org/10.1016/j.stemcr.2018.05.009

TY - JOUR

T1 - DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

AU - Park, Jongjin

AU - Son, Yeonsung

AU - Lee, Na Geum

AU - Lee, Kyungmin

AU - Lee, Dong Gwang

AU - Song, Jinhoi

AU - Lee, Jaemin

AU - Kim, Seokho

AU - Cho, Min Ji

AU - Jang, Ju Hong

AU - Lee, Jangwook

AU - Park, Jong Gil

AU - Kim, Yeon Gu

AU - Kim, Jang Seong

AU - Lee, Jungwoon

AU - Cho, Yee Sook

AU - Park, Young Jun

AU - Han, Baek Soo

AU - Bae, Kwang Hee

AU - Han, Seungmin

AU - Kang, Byunghoon

AU - Haam, Seungjoo

AU - Lee, Sang Hyun

AU - Lee, Sang Chul

AU - Min, Jeong Ki

N1 - Funding Information: This study was supported by a grant from the Korea Research Institute of Bioscience and Biotechnology and by grants from the National Research Foundation of Korea , which was funded by the Ministry of Science, Information & Communication Technology and Future Planning ( NRF-2012M3A9C6050331 , NRF-2015M3A9C6030284 , NRF-2015M3A9D7029882 , NRF-2013M3A9B6046566 , and NRF-2012M3A9C7050101 ). Publisher Copyright: © 2018 The Authors

PY - 2018/7/10

Y1 - 2018/7/10

N2 - Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

AB - Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

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ER -

DSG2 Is a Functional Cell Surface Marker for Identification and Isolation of Human Pluripotent Stem Cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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