Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation

Yu Chih Chen, Zhixiong Zhang, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Considerable evidence suggests that cancer-stromal interactions are critical in tumor pathogenesis, but conventional dish-based co-culture methods lack in capability of precisely controlling microenvironments and tracking single cell behavior. To better model tumorigenesis, we present a dual adherent/suspension co-culture device, which can provide both a suspension environment for cancer cells and an adherent environment for stromal cells in close proximity by selectively patterning polyHEMA in indented micro-wells. For investigating cellular heterogeneity, single tumor cells were monitored in a microwell surrounded by stromal cells. As the proof of concept, we have successfully demonstrated the elevated stemness and proliferation rate induced by cancer-stromal interactions.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages305-307
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Fingerprint

Tumors
Cells

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Chen, Y. C., Zhang, Z., & Yoon, E. (2015). Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 305-307). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.
Chen, Yu Chih ; Zhang, Zhixiong ; Yoon, Euisik. / Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 305-307 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
@inproceedings{618b88ce4a334e2e824d9b2a6de16777,
title = "Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation",
abstract = "Considerable evidence suggests that cancer-stromal interactions are critical in tumor pathogenesis, but conventional dish-based co-culture methods lack in capability of precisely controlling microenvironments and tracking single cell behavior. To better model tumorigenesis, we present a dual adherent/suspension co-culture device, which can provide both a suspension environment for cancer cells and an adherent environment for stromal cells in close proximity by selectively patterning polyHEMA in indented micro-wells. For investigating cellular heterogeneity, single tumor cells were monitored in a microwell surrounded by stromal cells. As the proof of concept, we have successfully demonstrated the elevated stemness and proliferation rate induced by cancer-stromal interactions.",
author = "Chen, {Yu Chih} and Zhixiong Zhang and Euisik Yoon",
year = "2015",
month = "1",
day = "1",
language = "English",
series = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "305--307",
booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Chen, YC, Zhang, Z & Yoon, E 2015, Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 305-307, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.

Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation. / Chen, Yu Chih; Zhang, Zhixiong; Yoon, Euisik.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 305-307 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation

AU - Chen, Yu Chih

AU - Zhang, Zhixiong

AU - Yoon, Euisik

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Considerable evidence suggests that cancer-stromal interactions are critical in tumor pathogenesis, but conventional dish-based co-culture methods lack in capability of precisely controlling microenvironments and tracking single cell behavior. To better model tumorigenesis, we present a dual adherent/suspension co-culture device, which can provide both a suspension environment for cancer cells and an adherent environment for stromal cells in close proximity by selectively patterning polyHEMA in indented micro-wells. For investigating cellular heterogeneity, single tumor cells were monitored in a microwell surrounded by stromal cells. As the proof of concept, we have successfully demonstrated the elevated stemness and proliferation rate induced by cancer-stromal interactions.

AB - Considerable evidence suggests that cancer-stromal interactions are critical in tumor pathogenesis, but conventional dish-based co-culture methods lack in capability of precisely controlling microenvironments and tracking single cell behavior. To better model tumorigenesis, we present a dual adherent/suspension co-culture device, which can provide both a suspension environment for cancer cells and an adherent environment for stromal cells in close proximity by selectively patterning polyHEMA in indented micro-wells. For investigating cellular heterogeneity, single tumor cells were monitored in a microwell surrounded by stromal cells. As the proof of concept, we have successfully demonstrated the elevated stemness and proliferation rate induced by cancer-stromal interactions.

UR - http://www.scopus.com/inward/record.url?scp=84983341679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983341679&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84983341679

T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 305

EP - 307

BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

ER -

Chen YC, Zhang Z, Yoon E. Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 305-307. (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).