Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation

Yu Chih Chen, Zhixiong Zhang, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Considerable evidence suggests that cancer-stromal interactions are critical in tumor pathogenesis, but conventional dish-based co-culture methods lack in capability of precisely controlling microenvironments and tracking single cell behavior. To better model tumorigenesis, we present a dual adherent/suspension co-culture device, which can provide both a suspension environment for cancer cells and an adherent environment for stromal cells in close proximity by selectively patterning polyHEMA in indented micro-wells. For investigating cellular heterogeneity, single tumor cells were monitored in a microwell surrounded by stromal cells. As the proof of concept, we have successfully demonstrated the elevated stemness and proliferation rate induced by cancer-stromal interactions.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages305-307
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Chen, Y. C., Zhang, Z., & Yoon, E. (2015). Dual adherent/suspension co-culture microenvironment for the study of cancer-stromal interactions using single-cell derived sphere formation. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 305-307). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.