Dual mode quantitative imaging of microscopic viscosity using a conjugated porphyrin dimer

Aurimas Vyšniauskas, Milan Balaz, Harry L. Anderson, Marina K. Kuimova

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Microviscosity is of paramount importance in materials and bio-sciences. Fluorescence imaging using molecular rotors has emerged as a versatile tool to measure microviscosity, either using a fluorescence lifetime or a ratiometric signal of the rotor; however, only a limited number of blue-to-green-emitting fluorophores with both the lifetime and the ratiometric signal sensitivity to viscosity have been reported to date. Here we report a deep red emitting dual viscosity sensor, which allows both the ratiometric and the lifetime imaging of viscosity. We study viscosity in a range of lipid-based systems and conclude that in complex dynamic systems dual detection is preferable in order to independently verify the results of the measurements as well as perform rapid detection of changing viscosity.

Original languageEnglish
Pages (from-to)7548-7554
Number of pages7
JournalPhysical Chemistry Chemical Physics
Volume17
Issue number11
DOIs
Publication statusPublished - 2015 Mar 21

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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