Dual pathways of p53 mediated glucolipotoxicity-induced apoptosis of rat cardiomyoblast cell: Activation of p53 proapoptosis and inhibition of Nrf2-NQO1 antiapoptosis

Hye Jin Wang, Eun Young Lee, Seung Jin Han, Soo Hyun Kim, Byung Wan Lee, Chul Woo Ahn, Bong Soo Cha, Hyun Chul Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reactive oxygen species (ROS), driven by excessive levels of glucose and free fatty acids, appears to induce cell apoptosis. However, the underlying molecular mechanism of this process remains unclear in cardiac myocytes. We investigated the glucolipotoxicity effects of high glucose and palmitic acid (C16:0) on the rat cardiomyoblast cell line (H9c2) focusing on tumor suppressor p53. Cultured H9c2 rat cardiomyoblasts were exposed to palmitate and /or to an elevated glucose concentration for 18 hours. Only the glucolipotoxic condition of 30 mM glucose in combination with 250μM palmitate resulted in significant generation of ROS and upregulation of p53 which caused to an increased cleavage of caspase-3. On the other hand, the expression of NF-E2-related factor 2 (Nrf2) showed increased tendency while the expression of NAD(P)H: quinone oxidoreductase-1 (NQO1) was decreased. N-acetyl L cysteins and pifithrin-α, an inhibitor of p53 abrogated glucolipotoxicity-induced ROS generation and p53 expression. Chromatin immunoprecipitation analysis revealed that p53 interacted antioxidant responsive elements (ARE)-containing promoter of NQO1. Upregulated p53 counteracted the Nrf2-induced transcription of ARE-containing promoter of NQO1 gene and leaded to decrease in NQO1 expression. We demonstrated that the elevated p53 mediated glucolipotoxicity-induced apoptosis of rat cardiomyoblast cell through dual pathways: stimulating pro-apoptosis signaling as well as suppressing anti-apoptosis pathway of Nrf2-NQO1 signaling.

Original languageEnglish
Pages (from-to)496-503
Number of pages8
JournalMetabolism: Clinical and Experimental
Volume61
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

NF-E2-Related Factor 2
Apoptosis
Glucose
Reactive Oxygen Species
Palmitates
Antioxidants
Palmitic Acid
Chromatin Immunoprecipitation
Nonesterified Fatty Acids
Cardiac Myocytes
Caspase 3
NAD
Oxidoreductases
Up-Regulation
Cell Line
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Wang, Hye Jin ; Lee, Eun Young ; Han, Seung Jin ; Kim, Soo Hyun ; Lee, Byung Wan ; Ahn, Chul Woo ; Cha, Bong Soo ; Lee, Hyun Chul. / Dual pathways of p53 mediated glucolipotoxicity-induced apoptosis of rat cardiomyoblast cell : Activation of p53 proapoptosis and inhibition of Nrf2-NQO1 antiapoptosis. In: Metabolism: Clinical and Experimental. 2012 ; Vol. 61, No. 4. pp. 496-503.
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abstract = "Reactive oxygen species (ROS), driven by excessive levels of glucose and free fatty acids, appears to induce cell apoptosis. However, the underlying molecular mechanism of this process remains unclear in cardiac myocytes. We investigated the glucolipotoxicity effects of high glucose and palmitic acid (C16:0) on the rat cardiomyoblast cell line (H9c2) focusing on tumor suppressor p53. Cultured H9c2 rat cardiomyoblasts were exposed to palmitate and /or to an elevated glucose concentration for 18 hours. Only the glucolipotoxic condition of 30 mM glucose in combination with 250μM palmitate resulted in significant generation of ROS and upregulation of p53 which caused to an increased cleavage of caspase-3. On the other hand, the expression of NF-E2-related factor 2 (Nrf2) showed increased tendency while the expression of NAD(P)H: quinone oxidoreductase-1 (NQO1) was decreased. N-acetyl L cysteins and pifithrin-α, an inhibitor of p53 abrogated glucolipotoxicity-induced ROS generation and p53 expression. Chromatin immunoprecipitation analysis revealed that p53 interacted antioxidant responsive elements (ARE)-containing promoter of NQO1. Upregulated p53 counteracted the Nrf2-induced transcription of ARE-containing promoter of NQO1 gene and leaded to decrease in NQO1 expression. We demonstrated that the elevated p53 mediated glucolipotoxicity-induced apoptosis of rat cardiomyoblast cell through dual pathways: stimulating pro-apoptosis signaling as well as suppressing anti-apoptosis pathway of Nrf2-NQO1 signaling.",
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Dual pathways of p53 mediated glucolipotoxicity-induced apoptosis of rat cardiomyoblast cell : Activation of p53 proapoptosis and inhibition of Nrf2-NQO1 antiapoptosis. / Wang, Hye Jin; Lee, Eun Young; Han, Seung Jin; Kim, Soo Hyun; Lee, Byung Wan; Ahn, Chul Woo; Cha, Bong Soo; Lee, Hyun Chul.

In: Metabolism: Clinical and Experimental, Vol. 61, No. 4, 01.04.2012, p. 496-503.

Research output: Contribution to journalArticle

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AU - Wang, Hye Jin

AU - Lee, Eun Young

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AU - Kim, Soo Hyun

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AU - Lee, Hyun Chul

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