Background. To evaluate the diagnostic value of dual priming oligonucleotide (DPO) - based multiplex polymerase chain reaction (PCR) for the detection of BRAFV600E mutations in ultrasound-guided fine-needle aspiration biopsy (USFNAB) of thyroid nodules. Methods. Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPObased multiplex PCR, direct DNA sequencing, and PCR - restriction fragment length polymorphism (RFLP) were used to detect BRAFV600E. The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. Results. Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAFV600E positivity was a useful marker at thyroid nodules with "suspicious for papillary thyroid carcinoma" or " inadequate" cytological result. Conclusions. DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAFV600E may be a useful additional diagnostic marker in BRAFV600E-prevalent areas.
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