Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAF V600E mutation-prevalent area

jinyoung kwak, Eunkyung Kim, Jong Kee Kim, Jeong Hyun Han, Soon Won Hong, Tae Sung Park, Jong Rak Choi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Background. To evaluate the diagnostic value of dual priming oligonucleotide (DPO) - based multiplex polymerase chain reaction (PCR) for the detection of BRAFV600E mutations in ultrasound-guided fine-needle aspiration biopsy (USFNAB) of thyroid nodules. Methods. Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPObased multiplex PCR, direct DNA sequencing, and PCR - restriction fragment length polymorphism (RFLP) were used to detect BRAFV600E. The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. Results. Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAFV600E positivity was a useful marker at thyroid nodules with "suspicious for papillary thyroid carcinoma" or " inadequate" cytological result. Conclusions. DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAFV600E may be a useful additional diagnostic marker in BRAFV600E-prevalent areas.

Original languageEnglish
Pages (from-to)490-498
Number of pages9
JournalHead and Neck
Volume32
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

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Thyroid Nodule
Multiplex Polymerase Chain Reaction
Oligonucleotides
Mutation
DNA Sequence Analysis
Research Ethics Committees
DNA-Directed DNA Polymerase
Fine Needle Biopsy
Informed Consent
Restriction Fragment Length Polymorphisms
Retrospective Studies
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

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title = "Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAF V600E mutation-prevalent area",
abstract = "Background. To evaluate the diagnostic value of dual priming oligonucleotide (DPO) - based multiplex polymerase chain reaction (PCR) for the detection of BRAFV600E mutations in ultrasound-guided fine-needle aspiration biopsy (USFNAB) of thyroid nodules. Methods. Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPObased multiplex PCR, direct DNA sequencing, and PCR - restriction fragment length polymorphism (RFLP) were used to detect BRAFV600E. The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. Results. Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAFV600E positivity was a useful marker at thyroid nodules with {"}suspicious for papillary thyroid carcinoma{"} or {"} inadequate{"} cytological result. Conclusions. DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAFV600E may be a useful additional diagnostic marker in BRAFV600E-prevalent areas.",
author = "jinyoung kwak and Eunkyung Kim and Kim, {Jong Kee} and Han, {Jeong Hyun} and Hong, {Soon Won} and Park, {Tae Sung} and Choi, {Jong Rak}",
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Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAF V600E mutation-prevalent area. / kwak, jinyoung; Kim, Eunkyung; Kim, Jong Kee; Han, Jeong Hyun; Hong, Soon Won; Park, Tae Sung; Choi, Jong Rak.

In: Head and Neck, Vol. 32, No. 4, 01.04.2010, p. 490-498.

Research output: Contribution to journalArticle

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AU - kwak, jinyoung

AU - Kim, Eunkyung

AU - Kim, Jong Kee

AU - Han, Jeong Hyun

AU - Hong, Soon Won

AU - Park, Tae Sung

AU - Choi, Jong Rak

PY - 2010/4/1

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N2 - Background. To evaluate the diagnostic value of dual priming oligonucleotide (DPO) - based multiplex polymerase chain reaction (PCR) for the detection of BRAFV600E mutations in ultrasound-guided fine-needle aspiration biopsy (USFNAB) of thyroid nodules. Methods. Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPObased multiplex PCR, direct DNA sequencing, and PCR - restriction fragment length polymorphism (RFLP) were used to detect BRAFV600E. The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. Results. Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAFV600E positivity was a useful marker at thyroid nodules with "suspicious for papillary thyroid carcinoma" or " inadequate" cytological result. Conclusions. DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAFV600E may be a useful additional diagnostic marker in BRAFV600E-prevalent areas.

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