Dual requirement for Rho and protein kinase C in direct activation of phospholipase D1 through G protein-coupled receptor signaling

G. Du, Y. M. Altshuller, Y. Kim, Min Han Jung Min Han, Ho Ryu Sung Ho Ryu, A. J. Morris, M. A. Frohman

Research output: Contribution to journalArticle

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Abstract

G protein-coupled and tyrosine kinase receptor activation of phospholipase D1 (PLD1) play key roles in agonist-stimulated cellular responses such as regulated exocytosis, actin stress fiber formation, and alterations in cell morphology and motility. Protein Kinase C, ADP-ribosylation factor (ARF), and Rho family members activate PLD1 in vitro; however, the actions of the stimulators on PLD1 in vivo have been proposed to take place through indirect pathways. We have used the yeast split-hybrid system to generate PLD1 alleles that fail to bind to or to be activated by RhoA but that retain wild-type responses to ARF and PKC. These alleles then were employed in combination with alleles unresponsive to PKC or to both stimulators to examine the activation of PLD1 by G protein-coupled receptors. Our results demonstrate that direct stimulation of PLD1 in vivo by RhoA (and by PKC) is critical for significant PLD1 activation but that PLD1 subcellular localization and regulated phosphorylation occur independently of these stimulatory pathways.

Original languageEnglish
Pages (from-to)4359-4368
Number of pages10
JournalMolecular Biology of the Cell
Volume11
Issue number12
DOIs
Publication statusPublished - 2000 Jan 1

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rho-Associated Kinases
G-Protein-Coupled Receptors
Protein Kinase C
ADP-Ribosylation Factors
Alleles
Stress Fibers
phospholipase D1
Exocytosis
Receptor Protein-Tyrosine Kinases
GTP-Binding Proteins
Cell Movement
Actins
Yeasts
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Du, G. ; Altshuller, Y. M. ; Kim, Y. ; Jung Min Han, Min Han ; Sung Ho Ryu, Ho Ryu ; Morris, A. J. ; Frohman, M. A. / Dual requirement for Rho and protein kinase C in direct activation of phospholipase D1 through G protein-coupled receptor signaling. In: Molecular Biology of the Cell. 2000 ; Vol. 11, No. 12. pp. 4359-4368.
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Dual requirement for Rho and protein kinase C in direct activation of phospholipase D1 through G protein-coupled receptor signaling. / Du, G.; Altshuller, Y. M.; Kim, Y.; Jung Min Han, Min Han; Sung Ho Ryu, Ho Ryu; Morris, A. J.; Frohman, M. A.

In: Molecular Biology of the Cell, Vol. 11, No. 12, 01.01.2000, p. 4359-4368.

Research output: Contribution to journalArticle

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