Dyadobacter soli sp. nov., a starch-degrading bacterium isolated from farm soil

Myungjin Lee, Sung Geun Woo, Joonhong Park, Soon Ae Yoo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A Gram-negative, non-motile, aerobic bacterial strain, designated MJ20 T, was isolated from farm soil near Daejeon (South Korea) and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ20T belongs to the family Cytophagaceae, class Sphingobacteria, and was related most closely to Dyadobacter fermentans DSM 18053T (98.9% sequence similarity), Dyadobacter beijingensis JCM 14200T (98.0 %) and Dyadobacter ginsengisoli KCTC 12589T (96.4 %). The G+C content of the genomic DNA of strain MJ20T was 48.5 mol%. The detection of MK-7 as the predominant menaquinone and a fatty acid profile with summed feature 4 (C 16:1ω7c and/or iso-C15:0 2-OH), iso-C 15:0, C16:0 and C16:1ω5c as major components supported the affiliation of strain MJ20T to the genus Dyadobacter. The new isolate exhibited relatively low levels of DNA-DNA relatedness with respect to D. fermentans DSM 18053T (mean±SD of three determinations, 47±7%) and D. beijingensis JCM 14200T (38±8 %). On the basis of its phenotypic and genotypic properties together with phylogenetic distinctiveness, strain MJ20T (=KCTC 22481T =JCM 16232T) should be classified in the genus Dyadobacter as the type strain of a novel species, for which the name Dyadobacter soli sp. nov. is proposed.

Original languageEnglish
Pages (from-to)2577-2582
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume60
Issue number11
DOIs
Publication statusPublished - 2010 Nov 1

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Dyadobacter
starch
Starch
Soil
farm
Bacteria
DNA
Cytophagaceae
farms
bacterium
new species
Sphingobacterium
bacteria
Vitamin K 2
soil
Republic of Korea
Base Composition
rRNA Genes
relatedness
Names

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Dyadobacter soli sp. nov., a starch-degrading bacterium isolated from farm soil",
abstract = "A Gram-negative, non-motile, aerobic bacterial strain, designated MJ20 T, was isolated from farm soil near Daejeon (South Korea) and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ20T belongs to the family Cytophagaceae, class Sphingobacteria, and was related most closely to Dyadobacter fermentans DSM 18053T (98.9{\%} sequence similarity), Dyadobacter beijingensis JCM 14200T (98.0 {\%}) and Dyadobacter ginsengisoli KCTC 12589T (96.4 {\%}). The G+C content of the genomic DNA of strain MJ20T was 48.5 mol{\%}. The detection of MK-7 as the predominant menaquinone and a fatty acid profile with summed feature 4 (C 16:1ω7c and/or iso-C15:0 2-OH), iso-C 15:0, C16:0 and C16:1ω5c as major components supported the affiliation of strain MJ20T to the genus Dyadobacter. The new isolate exhibited relatively low levels of DNA-DNA relatedness with respect to D. fermentans DSM 18053T (mean±SD of three determinations, 47±7{\%}) and D. beijingensis JCM 14200T (38±8 {\%}). On the basis of its phenotypic and genotypic properties together with phylogenetic distinctiveness, strain MJ20T (=KCTC 22481T =JCM 16232T) should be classified in the genus Dyadobacter as the type strain of a novel species, for which the name Dyadobacter soli sp. nov. is proposed.",
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Dyadobacter soli sp. nov., a starch-degrading bacterium isolated from farm soil. / Lee, Myungjin; Woo, Sung Geun; Park, Joonhong; Yoo, Soon Ae.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 60, No. 11, 01.11.2010, p. 2577-2582.

Research output: Contribution to journalArticle

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