This study aimed to achieve continuous biohydrogen production from red algal biomass using a dynamic membrane bioreactor (DMBR). The DMBR was continuously fed with pretreated Echeuma spinosum containing 20 g/L hexose. The highest average hydrogen production rate (HPR) of 21.58 ± 1.59 L/L-d was observed at HRT 3 h, which was higher than previous reports for continuous H2 production from biomass feedstock. Metabolic flux analysis revealed that butyric acid and propionic acid were the major by-products of the H2-producing and H2-consuming pathways, respectively, of the algal biomass fermentation. Hydrogen consumption by propionic acid pathway could not be prevented completely by heat treatment. PICRUSt2 analysis predicted that Clostridium sp., Anaerostipes sp., and Caproiciproducens sp. might significantly contribute to the expression of both ferredoxin hydrogenase and propionate CoA-transferase. This study would provide the design and operational information on high-rate bioreactor for continuous hydrogen production using biomass.
|Publication status||Published - 2021 Nov|
Bibliographical noteFunding Information:
The research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science & ICT) (No. NRF- 2019M3E6A1103839 , 2020R1A2B5B02001757 ).
© 2021 Elsevier Ltd
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal