Dynamic rearrangement of telomeres during spermatogenesis in mice

Kentaro Tanemura; Atsuo Ogura; Cheolho Cheong; Hideo Gotoh; Kazuya Matsumoto; Eimei Sato; Yoshihiro Hayashi; Han Woong Lee; Takashi Kondo

doi:10.1016/j.ydbio.2005.02.025

Dynamic rearrangement of telomeres during spermatogenesis in mice

Kentaro Tanemura, Atsuo Ogura, Cheolho Cheong, Hideo Gotoh, Kazuya Matsumoto, Eimei Sato, Yoshihiro Hayashi, Han Woong Lee, Takashi Kondo

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Chromosomal structure within the nucleus influences various biological processes such as transcription and replication. Telomeres are located at the end of eukaryotic chromosomes and they can be a decisive factor for correct chromosomal positioning. To gain new insight into telomere dynamics, we examined telomere length and positional changes during spermatogenesis using improved fluorescence in situ hybridization (FISH) and in situ telomeric repeat amplification protocols (TRAP) on histological sections. FISH revealed telomere length and chromosome position within nuclei change dynamically. Telomere extension occurred during spermiogenesis. In situ TRAP analysis verified elevated telomerase activity in elongating spermatids. Together, these data show that elongated spermatids have longer telomeres than precursor spermatogenic cells. This observation indicates that telomere elongation in haploid cells occurs after meiosis and in the absence of genomic replication. Analyses of testes from telomerase null mice further support the significance of telomere dynamics during spermatogenesis and the existence of an alternative telomere extension pathway.

Original language	English
Pages (from-to)	196-207
Number of pages	12
Journal	Developmental Biology
Volume	281
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2005.02.025
Publication status	Published - 2005 May 15

Bibliographical note

Funding Information:
We thank R.A. DePinho for providing the Terc knockout mice and the critical reading of the manuscript. We are grateful to S. Gasser for the critical reading of the manuscript and to the members of the Kondo Laboratory for sharing reagents. T.K. also would like to thank D. Duboule and M. Muramatsu for continuous encouragement, and the late G. Matsumoto for help in establishing the laboratory. T.K. is supported by a grant from the Human Frontier Scientific Program Organization (HFSPO) and Grant-in-Aid for Scientific Research on Priority Areas from The Ministry of Education, Culture, Sports, and Technology (MEXT) of Japan. K.T. is also funded by Grant-in-Aid for Scientific Research on Priority Areas from MEXT. Correspondence and requests for materials should be addressed to T.K. ( TKondo@brain.riken.go.jp ).

All Science Journal Classification (ASJC) codes

Molecular Biology
Developmental Biology
Cell Biology

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title = "Dynamic rearrangement of telomeres during spermatogenesis in mice",

abstract = "Chromosomal structure within the nucleus influences various biological processes such as transcription and replication. Telomeres are located at the end of eukaryotic chromosomes and they can be a decisive factor for correct chromosomal positioning. To gain new insight into telomere dynamics, we examined telomere length and positional changes during spermatogenesis using improved fluorescence in situ hybridization (FISH) and in situ telomeric repeat amplification protocols (TRAP) on histological sections. FISH revealed telomere length and chromosome position within nuclei change dynamically. Telomere extension occurred during spermiogenesis. In situ TRAP analysis verified elevated telomerase activity in elongating spermatids. Together, these data show that elongated spermatids have longer telomeres than precursor spermatogenic cells. This observation indicates that telomere elongation in haploid cells occurs after meiosis and in the absence of genomic replication. Analyses of testes from telomerase null mice further support the significance of telomere dynamics during spermatogenesis and the existence of an alternative telomere extension pathway.",

author = "Kentaro Tanemura and Atsuo Ogura and Cheolho Cheong and Hideo Gotoh and Kazuya Matsumoto and Eimei Sato and Yoshihiro Hayashi and Lee, {Han Woong} and Takashi Kondo",

note = "Funding Information: We thank R.A. DePinho for providing the Terc knockout mice and the critical reading of the manuscript. We are grateful to S. Gasser for the critical reading of the manuscript and to the members of the Kondo Laboratory for sharing reagents. T.K. also would like to thank D. Duboule and M. Muramatsu for continuous encouragement, and the late G. Matsumoto for help in establishing the laboratory. T.K. is supported by a grant from the Human Frontier Scientific Program Organization (HFSPO) and Grant-in-Aid for Scientific Research on Priority Areas from The Ministry of Education, Culture, Sports, and Technology (MEXT) of Japan. K.T. is also funded by Grant-in-Aid for Scientific Research on Priority Areas from MEXT. Correspondence and requests for materials should be addressed to T.K. ( TKondo@brain.riken.go.jp ).",

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AU - Tanemura, Kentaro

AU - Ogura, Atsuo

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AU - Sato, Eimei

AU - Hayashi, Yoshihiro

AU - Lee, Han Woong

AU - Kondo, Takashi

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N2 - Chromosomal structure within the nucleus influences various biological processes such as transcription and replication. Telomeres are located at the end of eukaryotic chromosomes and they can be a decisive factor for correct chromosomal positioning. To gain new insight into telomere dynamics, we examined telomere length and positional changes during spermatogenesis using improved fluorescence in situ hybridization (FISH) and in situ telomeric repeat amplification protocols (TRAP) on histological sections. FISH revealed telomere length and chromosome position within nuclei change dynamically. Telomere extension occurred during spermiogenesis. In situ TRAP analysis verified elevated telomerase activity in elongating spermatids. Together, these data show that elongated spermatids have longer telomeres than precursor spermatogenic cells. This observation indicates that telomere elongation in haploid cells occurs after meiosis and in the absence of genomic replication. Analyses of testes from telomerase null mice further support the significance of telomere dynamics during spermatogenesis and the existence of an alternative telomere extension pathway.

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Dynamic rearrangement of telomeres during spermatogenesis in mice

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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