Dynamic rearrangement of telomeres during spermatogenesis in mice

Kentaro Tanemura, Atsuo Ogura, Cheolho Cheong, Hideo Gotoh, Kazuya Matsumoto, Eimei Sato, Yoshihiro Hayashi, Han Woong Lee, Takashi Kondo

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Chromosomal structure within the nucleus influences various biological processes such as transcription and replication. Telomeres are located at the end of eukaryotic chromosomes and they can be a decisive factor for correct chromosomal positioning. To gain new insight into telomere dynamics, we examined telomere length and positional changes during spermatogenesis using improved fluorescence in situ hybridization (FISH) and in situ telomeric repeat amplification protocols (TRAP) on histological sections. FISH revealed telomere length and chromosome position within nuclei change dynamically. Telomere extension occurred during spermiogenesis. In situ TRAP analysis verified elevated telomerase activity in elongating spermatids. Together, these data show that elongated spermatids have longer telomeres than precursor spermatogenic cells. This observation indicates that telomere elongation in haploid cells occurs after meiosis and in the absence of genomic replication. Analyses of testes from telomerase null mice further support the significance of telomere dynamics during spermatogenesis and the existence of an alternative telomere extension pathway.

Original languageEnglish
Pages (from-to)196-207
Number of pages12
JournalDevelopmental Biology
Volume281
Issue number2
DOIs
Publication statusPublished - 2005 May 15

Fingerprint

Telomere
Spermatogenesis
Spermatids
Telomerase
Fluorescence In Situ Hybridization
Chromosomes
Biological Phenomena
Haploidy
Meiosis
In Situ Hybridization
Testis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Tanemura, K., Ogura, A., Cheong, C., Gotoh, H., Matsumoto, K., Sato, E., ... Kondo, T. (2005). Dynamic rearrangement of telomeres during spermatogenesis in mice. Developmental Biology, 281(2), 196-207. https://doi.org/10.1016/j.ydbio.2005.02.025
Tanemura, Kentaro ; Ogura, Atsuo ; Cheong, Cheolho ; Gotoh, Hideo ; Matsumoto, Kazuya ; Sato, Eimei ; Hayashi, Yoshihiro ; Lee, Han Woong ; Kondo, Takashi. / Dynamic rearrangement of telomeres during spermatogenesis in mice. In: Developmental Biology. 2005 ; Vol. 281, No. 2. pp. 196-207.
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Tanemura, K, Ogura, A, Cheong, C, Gotoh, H, Matsumoto, K, Sato, E, Hayashi, Y, Lee, HW & Kondo, T 2005, 'Dynamic rearrangement of telomeres during spermatogenesis in mice', Developmental Biology, vol. 281, no. 2, pp. 196-207. https://doi.org/10.1016/j.ydbio.2005.02.025

Dynamic rearrangement of telomeres during spermatogenesis in mice. / Tanemura, Kentaro; Ogura, Atsuo; Cheong, Cheolho; Gotoh, Hideo; Matsumoto, Kazuya; Sato, Eimei; Hayashi, Yoshihiro; Lee, Han Woong; Kondo, Takashi.

In: Developmental Biology, Vol. 281, No. 2, 15.05.2005, p. 196-207.

Research output: Contribution to journalArticle

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Tanemura K, Ogura A, Cheong C, Gotoh H, Matsumoto K, Sato E et al. Dynamic rearrangement of telomeres during spermatogenesis in mice. Developmental Biology. 2005 May 15;281(2):196-207. https://doi.org/10.1016/j.ydbio.2005.02.025