E.coli outer membrane with autodisplayed Z-domain as a molecular recognition layer of SPR biosensor

M. Park, J. Jose, Jae Chul Pyun

Research output: Contribution to journalConference article

Abstract

In this work, Z domain of protein A with IgG-binding activity was expressed on the outer membrane of E.coli as a fusion protein of AIDA-1 by using Autodisplay method, and the outer membrane with autodisplayed Z-domain was applied for the SPR biosensor. The outer membrane of E.coli with Z-domain was coated to the gold surface of SPR biosensor. After anti-hIgG antibodies were immobilized to the Z-domain, hIgG antibodies were measured as a target analyte. The LOD of SPR biosensor with the outer membrane of E.coli with Z-domain and intact E.coli outer membrane was estimated to be 1.6 ng/ml, 316 ng/ml, respectively. This result shows that the outer membrane of E.coli with Z-domain could increased the sensitivity of SPR biosensor through the orientation control of antibodies at the molecular recognition layer.

Original languageEnglish
Pages (from-to)1475-1478
Number of pages4
JournalProcedia Chemistry
Volume1
Issue number1
DOIs
Publication statusPublished - 2009 Sep 1
EventEurosensors 23rd Conference - Lausanne, Switzerland
Duration: 2009 Sep 62009 Sep 9

Fingerprint

Molecular recognition
Biosensors
Escherichia coli
Membranes
Antibodies
Proteins
Staphylococcal Protein A
Gold
Fusion reactions
Immunoglobulin G

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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abstract = "In this work, Z domain of protein A with IgG-binding activity was expressed on the outer membrane of E.coli as a fusion protein of AIDA-1 by using Autodisplay method, and the outer membrane with autodisplayed Z-domain was applied for the SPR biosensor. The outer membrane of E.coli with Z-domain was coated to the gold surface of SPR biosensor. After anti-hIgG antibodies were immobilized to the Z-domain, hIgG antibodies were measured as a target analyte. The LOD of SPR biosensor with the outer membrane of E.coli with Z-domain and intact E.coli outer membrane was estimated to be 1.6 ng/ml, 316 ng/ml, respectively. This result shows that the outer membrane of E.coli with Z-domain could increased the sensitivity of SPR biosensor through the orientation control of antibodies at the molecular recognition layer.",
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E.coli outer membrane with autodisplayed Z-domain as a molecular recognition layer of SPR biosensor. / Park, M.; Jose, J.; Pyun, Jae Chul.

In: Procedia Chemistry, Vol. 1, No. 1, 01.09.2009, p. 1475-1478.

Research output: Contribution to journalConference article

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AU - Jose, J.

AU - Pyun, Jae Chul

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AB - In this work, Z domain of protein A with IgG-binding activity was expressed on the outer membrane of E.coli as a fusion protein of AIDA-1 by using Autodisplay method, and the outer membrane with autodisplayed Z-domain was applied for the SPR biosensor. The outer membrane of E.coli with Z-domain was coated to the gold surface of SPR biosensor. After anti-hIgG antibodies were immobilized to the Z-domain, hIgG antibodies were measured as a target analyte. The LOD of SPR biosensor with the outer membrane of E.coli with Z-domain and intact E.coli outer membrane was estimated to be 1.6 ng/ml, 316 ng/ml, respectively. This result shows that the outer membrane of E.coli with Z-domain could increased the sensitivity of SPR biosensor through the orientation control of antibodies at the molecular recognition layer.

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