Ecological changes in oral microcosm biofilm during maturation

Young Seok Kim, Si Mook Kang, Eun Song Lee, Ji Hyun Lee, Bo Ra Kim, Baek Il Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria.

Original languageEnglish
Article number101409
JournalJournal of Biomedical Optics
Volume21
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

biofilms
Biofilms
Fluorescence
fluorescence
bacteria
Bacteria
saliva
sequencing
Mucins
Ribosomal DNA
Chemical analysis
genes
Genes
slopes
Monitoring

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Kim, Young Seok ; Kang, Si Mook ; Lee, Eun Song ; Lee, Ji Hyun ; Kim, Bo Ra ; Kim, Baek Il. / Ecological changes in oral microcosm biofilm during maturation. In: Journal of Biomedical Optics. 2016 ; Vol. 21, No. 10.
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Ecological changes in oral microcosm biofilm during maturation. / Kim, Young Seok; Kang, Si Mook; Lee, Eun Song; Lee, Ji Hyun; Kim, Bo Ra; Kim, Baek Il.

In: Journal of Biomedical Optics, Vol. 21, No. 10, 101409, 01.10.2016.

Research output: Contribution to journalArticle

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