Ectopic expression of apple fruit homogentisate phytyltransferase gene (MdHPT1) increases tocopherol in transgenic tomato (Solanum lycopersicum cv. Micro-Tom) leaves and fruits

Young Sam Seo; Soo Jin Kim; Chee Hark Harn; Woo Taek Kim

doi:10.1016/j.phytochem.2010.12.013

Ectopic expression of apple fruit homogentisate phytyltransferase gene (MdHPT1) increases tocopherol in transgenic tomato (Solanum lycopersicum cv. Micro-Tom) leaves and fruits

Young Sam Seo, Soo Jin Kim, Chee Hark Harn, Woo Taek Kim

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Homogentisate phytyltransferase (HPT) is an important enzyme in the biosynthesis of tocopherols (vitamin E). Herein, an HPT homolog (MdHPT1) was isolated from apple (Malus domestica Borkh. cv. Fuji) fruits, whose gene expression level gradually decreased during fruit ripening, reaching a background level in ripened apple fruits. The amounts of α- and γ-tocopherols, two major tocopherols in plant organs, were 5- to 14-fold lower in the fruits than in the leaves and flowers of apple plants. Transgenic tomato plants (Solanum lycopersicum cv. Micro-Tom) overexpressing MdHPT1 were next constructed. Transgenic independent T₁ leaves contained ∼1.8- to 3.6-fold and ∼1.6- to 2.9-fold higher levels of α-tocopherol and γ-tocopherol, respectively, than those in control plants. In addition, the levels of α-tocopherol and γ-tocopherol in 35S:MdHPT1 T₁ fruits increased up to 1.7-fold and 3.1-fold, respectively, as compared to the control fruits, indicating that an increase in α-tocopherol in fruits (maximal 1.7-fold) was less evident than that in leaves (maximal 3.6-fold). This finding suggests that the apple MdHPT1 plays a role in tocopherol production in transgenic tomatoes.

Original language	English
Pages (from-to)	321-329
Number of pages	9
Journal	Phytochemistry
Volume	72
Issue number	4-5
DOIs	https://doi.org/10.1016/j.phytochem.2010.12.013
Publication status	Published - 2011 Apr

Bibliographical note

Funding Information:
We are thankful to Dr. Myung Hee Nam and Ms. Joo Hee Chung at the Korea Basic Science Institute (Seoul, Korea) for their help and discussion about tocopherol analysis. This work was supported by grants from the BioGreen 21 Program (funded by the Rural Development Administration ), the Technology Development Program for Agriculture and Forestry (Project No. 309017-5 funded by the Ministry for Agriculture, Forestry and Fisheries, Republic of Korea ), and the National Research Foundation (Project No. 2009-0078317 funded by the Ministry of Education, Science, and Technology, Republic of Korea ).

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/j.phytochem.2010.12.013

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@article{6692b810afe3415890cc3425f3e9f29e,

title = "Ectopic expression of apple fruit homogentisate phytyltransferase gene (MdHPT1) increases tocopherol in transgenic tomato (Solanum lycopersicum cv. Micro-Tom) leaves and fruits",

abstract = "Homogentisate phytyltransferase (HPT) is an important enzyme in the biosynthesis of tocopherols (vitamin E). Herein, an HPT homolog (MdHPT1) was isolated from apple (Malus domestica Borkh. cv. Fuji) fruits, whose gene expression level gradually decreased during fruit ripening, reaching a background level in ripened apple fruits. The amounts of α- and γ-tocopherols, two major tocopherols in plant organs, were 5- to 14-fold lower in the fruits than in the leaves and flowers of apple plants. Transgenic tomato plants (Solanum lycopersicum cv. Micro-Tom) overexpressing MdHPT1 were next constructed. Transgenic independent T1 leaves contained ∼1.8- to 3.6-fold and ∼1.6- to 2.9-fold higher levels of α-tocopherol and γ-tocopherol, respectively, than those in control plants. In addition, the levels of α-tocopherol and γ-tocopherol in 35S:MdHPT1 T1 fruits increased up to 1.7-fold and 3.1-fold, respectively, as compared to the control fruits, indicating that an increase in α-tocopherol in fruits (maximal 1.7-fold) was less evident than that in leaves (maximal 3.6-fold). This finding suggests that the apple MdHPT1 plays a role in tocopherol production in transgenic tomatoes.",

author = "Seo, {Young Sam} and Kim, {Soo Jin} and Harn, {Chee Hark} and Kim, {Woo Taek}",

note = "Funding Information: We are thankful to Dr. Myung Hee Nam and Ms. Joo Hee Chung at the Korea Basic Science Institute (Seoul, Korea) for their help and discussion about tocopherol analysis. This work was supported by grants from the BioGreen 21 Program (funded by the Rural Development Administration ), the Technology Development Program for Agriculture and Forestry (Project No. 309017-5 funded by the Ministry for Agriculture, Forestry and Fisheries, Republic of Korea ), and the National Research Foundation (Project No. 2009-0078317 funded by the Ministry of Education, Science, and Technology, Republic of Korea ).",

year = "2011",

month = apr,

doi = "10.1016/j.phytochem.2010.12.013",

language = "English",

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AU - Seo, Young Sam

AU - Kim, Soo Jin

AU - Harn, Chee Hark

AU - Kim, Woo Taek

N1 - Funding Information: We are thankful to Dr. Myung Hee Nam and Ms. Joo Hee Chung at the Korea Basic Science Institute (Seoul, Korea) for their help and discussion about tocopherol analysis. This work was supported by grants from the BioGreen 21 Program (funded by the Rural Development Administration ), the Technology Development Program for Agriculture and Forestry (Project No. 309017-5 funded by the Ministry for Agriculture, Forestry and Fisheries, Republic of Korea ), and the National Research Foundation (Project No. 2009-0078317 funded by the Ministry of Education, Science, and Technology, Republic of Korea ).

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N2 - Homogentisate phytyltransferase (HPT) is an important enzyme in the biosynthesis of tocopherols (vitamin E). Herein, an HPT homolog (MdHPT1) was isolated from apple (Malus domestica Borkh. cv. Fuji) fruits, whose gene expression level gradually decreased during fruit ripening, reaching a background level in ripened apple fruits. The amounts of α- and γ-tocopherols, two major tocopherols in plant organs, were 5- to 14-fold lower in the fruits than in the leaves and flowers of apple plants. Transgenic tomato plants (Solanum lycopersicum cv. Micro-Tom) overexpressing MdHPT1 were next constructed. Transgenic independent T1 leaves contained ∼1.8- to 3.6-fold and ∼1.6- to 2.9-fold higher levels of α-tocopherol and γ-tocopherol, respectively, than those in control plants. In addition, the levels of α-tocopherol and γ-tocopherol in 35S:MdHPT1 T1 fruits increased up to 1.7-fold and 3.1-fold, respectively, as compared to the control fruits, indicating that an increase in α-tocopherol in fruits (maximal 1.7-fold) was less evident than that in leaves (maximal 3.6-fold). This finding suggests that the apple MdHPT1 plays a role in tocopherol production in transgenic tomatoes.

AB - Homogentisate phytyltransferase (HPT) is an important enzyme in the biosynthesis of tocopherols (vitamin E). Herein, an HPT homolog (MdHPT1) was isolated from apple (Malus domestica Borkh. cv. Fuji) fruits, whose gene expression level gradually decreased during fruit ripening, reaching a background level in ripened apple fruits. The amounts of α- and γ-tocopherols, two major tocopherols in plant organs, were 5- to 14-fold lower in the fruits than in the leaves and flowers of apple plants. Transgenic tomato plants (Solanum lycopersicum cv. Micro-Tom) overexpressing MdHPT1 were next constructed. Transgenic independent T1 leaves contained ∼1.8- to 3.6-fold and ∼1.6- to 2.9-fold higher levels of α-tocopherol and γ-tocopherol, respectively, than those in control plants. In addition, the levels of α-tocopherol and γ-tocopherol in 35S:MdHPT1 T1 fruits increased up to 1.7-fold and 3.1-fold, respectively, as compared to the control fruits, indicating that an increase in α-tocopherol in fruits (maximal 1.7-fold) was less evident than that in leaves (maximal 3.6-fold). This finding suggests that the apple MdHPT1 plays a role in tocopherol production in transgenic tomatoes.

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Ectopic expression of apple fruit homogentisate phytyltransferase gene (MdHPT1) increases tocopherol in transgenic tomato (Solanum lycopersicum cv. Micro-Tom) leaves and fruits

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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