Effect of β-caryophyllene from cloves extract on helicobacter pylori eradication in mouse model

Da Hyun Jung; Mi Hee Park; Chul Jin Kim; Jin Young Lee; Chang Yeop Keum; In Seon Kim; Chang Hyun Yun; Sung Kyu Kim; Won Ho Kim; Yong Chan Lee

doi:10.3390/nu12041000

Effect of β-caryophyllene from cloves extract on helicobacter pylori eradication in mouse model

Da Hyun Jung, Mi Hee Park, Chul Jin Kim, Jin Young Lee, Chang Yeop Keum, In Seon Kim, Chang Hyun Yun, Sung Kyu Kim, Won Ho Kim, Yong Chan Lee

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

New antibacterial treatments against Helicobacter pylori are needed as H. pylori is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-002119-00-001 from β-caryophyllene on the eradication of H. pylori in a mouse model, and its effects on the inflammation of the gastric mucosa. To evaluate the anti-H.pylori efficacy of β-caryophyllene, a total of 160 mice were divided into eight groups (n = 10 each) and were administered different treatments for 2 and 4 weeks. H. pylori eradication was assessed using a Campylobacter-like organism (CLO) test and H. pylori qPCR of the gastric mucosa. The levels of inflammation of gastric mucosa were assessed using histology and immunostaining. H-002119-00-001 decreased bacterial burden in vitro. When H-002119-00-001 was administered to mice once daily for 2 weeks, cure rates shown by the CLO test were 40.0%, 60.0%, and 70.0% in groups 6, 7, and 8, respectively. H. pylori levels in gastric mucosa decreased dose-dependently after H-002119-00-001 treatment. H-002119-00-001 also reduced levels of inflammation in gastric mucosa. H-002119-00-001 improved inflammation and decreased bacterial burden in H. pylori-infected mouse models. H-002119-00-001 is a promising and effective therapeutic agent for the treatment of H. pylori infection.

Original language	English
Article number	1000
Journal	Nutrients
Volume	12
Issue number	4
DOIs	https://doi.org/10.3390/nu12041000
Publication status	Published - 2020 Apr

Bibliographical note

Funding Information:
This work was supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Enterprise Open-Innovative Voucher Program (R&D, grant number P0010753), supervised by the Korea Institute for Advancement of Technology (KIAT).

Funding Information:
Funding: This work was supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Enterprise Open-Innovative Voucher Program (R&D, grant number P0010753), supervised by the Korea Institute for Advancement of Technology (KIAT).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Food Science
Nutrition and Dietetics

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10.3390/nu12041000

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@article{5b55c9d974d34edab0f395ba7e10ebe6,

title = "Effect of β-caryophyllene from cloves extract on helicobacter pylori eradication in mouse model",

abstract = "New antibacterial treatments against Helicobacter pylori are needed as H. pylori is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-002119-00-001 from β-caryophyllene on the eradication of H. pylori in a mouse model, and its effects on the inflammation of the gastric mucosa. To evaluate the anti-H.pylori efficacy of β-caryophyllene, a total of 160 mice were divided into eight groups (n = 10 each) and were administered different treatments for 2 and 4 weeks. H. pylori eradication was assessed using a Campylobacter-like organism (CLO) test and H. pylori qPCR of the gastric mucosa. The levels of inflammation of gastric mucosa were assessed using histology and immunostaining. H-002119-00-001 decreased bacterial burden in vitro. When H-002119-00-001 was administered to mice once daily for 2 weeks, cure rates shown by the CLO test were 40.0%, 60.0%, and 70.0% in groups 6, 7, and 8, respectively. H. pylori levels in gastric mucosa decreased dose-dependently after H-002119-00-001 treatment. H-002119-00-001 also reduced levels of inflammation in gastric mucosa. H-002119-00-001 improved inflammation and decreased bacterial burden in H. pylori-infected mouse models. H-002119-00-001 is a promising and effective therapeutic agent for the treatment of H. pylori infection.",

author = "Jung, {Da Hyun} and Park, {Mi Hee} and Kim, {Chul Jin} and Lee, {Jin Young} and Keum, {Chang Yeop} and Kim, {In Seon} and Yun, {Chang Hyun} and Kim, {Sung Kyu} and Kim, {Won Ho} and Lee, {Yong Chan}",

note = "Funding Information: This work was supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Enterprise Open-Innovative Voucher Program (R&D, grant number P0010753), supervised by the Korea Institute for Advancement of Technology (KIAT). Funding Information: Funding: This work was supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Enterprise Open-Innovative Voucher Program (R&D, grant number P0010753), supervised by the Korea Institute for Advancement of Technology (KIAT). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = apr,

doi = "10.3390/nu12041000",

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T1 - Effect of β-caryophyllene from cloves extract on helicobacter pylori eradication in mouse model

AU - Jung, Da Hyun

AU - Park, Mi Hee

AU - Kim, Chul Jin

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AU - Keum, Chang Yeop

AU - Kim, In Seon

AU - Yun, Chang Hyun

AU - Kim, Sung Kyu

AU - Kim, Won Ho

AU - Lee, Yong Chan

N1 - Funding Information: This work was supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Enterprise Open-Innovative Voucher Program (R&D, grant number P0010753), supervised by the Korea Institute for Advancement of Technology (KIAT). Funding Information: Funding: This work was supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the Regional Enterprise Open-Innovative Voucher Program (R&D, grant number P0010753), supervised by the Korea Institute for Advancement of Technology (KIAT). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/4

Y1 - 2020/4

N2 - New antibacterial treatments against Helicobacter pylori are needed as H. pylori is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-002119-00-001 from β-caryophyllene on the eradication of H. pylori in a mouse model, and its effects on the inflammation of the gastric mucosa. To evaluate the anti-H.pylori efficacy of β-caryophyllene, a total of 160 mice were divided into eight groups (n = 10 each) and were administered different treatments for 2 and 4 weeks. H. pylori eradication was assessed using a Campylobacter-like organism (CLO) test and H. pylori qPCR of the gastric mucosa. The levels of inflammation of gastric mucosa were assessed using histology and immunostaining. H-002119-00-001 decreased bacterial burden in vitro. When H-002119-00-001 was administered to mice once daily for 2 weeks, cure rates shown by the CLO test were 40.0%, 60.0%, and 70.0% in groups 6, 7, and 8, respectively. H. pylori levels in gastric mucosa decreased dose-dependently after H-002119-00-001 treatment. H-002119-00-001 also reduced levels of inflammation in gastric mucosa. H-002119-00-001 improved inflammation and decreased bacterial burden in H. pylori-infected mouse models. H-002119-00-001 is a promising and effective therapeutic agent for the treatment of H. pylori infection.

AB - New antibacterial treatments against Helicobacter pylori are needed as H. pylori is acquiring antibiotic resistance. β-caryophyllene is a natural bicyclic sesquiterpene, with anti-inflammatory and antimicrobial effects. This study investigates the effects of H-002119-00-001 from β-caryophyllene on the eradication of H. pylori in a mouse model, and its effects on the inflammation of the gastric mucosa. To evaluate the anti-H.pylori efficacy of β-caryophyllene, a total of 160 mice were divided into eight groups (n = 10 each) and were administered different treatments for 2 and 4 weeks. H. pylori eradication was assessed using a Campylobacter-like organism (CLO) test and H. pylori qPCR of the gastric mucosa. The levels of inflammation of gastric mucosa were assessed using histology and immunostaining. H-002119-00-001 decreased bacterial burden in vitro. When H-002119-00-001 was administered to mice once daily for 2 weeks, cure rates shown by the CLO test were 40.0%, 60.0%, and 70.0% in groups 6, 7, and 8, respectively. H. pylori levels in gastric mucosa decreased dose-dependently after H-002119-00-001 treatment. H-002119-00-001 also reduced levels of inflammation in gastric mucosa. H-002119-00-001 improved inflammation and decreased bacterial burden in H. pylori-infected mouse models. H-002119-00-001 is a promising and effective therapeutic agent for the treatment of H. pylori infection.

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Effect of β-caryophyllene from cloves extract on helicobacter pylori eradication in mouse model

Abstract

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