Effect of 1-deoxynojirimycin on cholesterol efflux through ABCA1-LXRα pathway in 3T3-L1 adipocytes

Hyun Ju Do, Seung-Min Lee, Young Soon Kim, Min Jeong Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We aimed to investigate whether 1-deoxynojirimycin (DNJ) has effects on cholesterol efflux in adipocytes and modulates ABCA1 expression in high fat fed mice. Fully differentiated 3T3-L1 adipocytes were tested for the cytotoxicity of DNJ and cholesterol efflux into apoA1/HDL was then assessed. ABCA1 protein levels and nuclear abundance of LXRα were measured after the treatment of DNJ in differentiated 3T3-L1 adipocytes. The effects of 12. week DNJ supplementation on ABCA1 gene expression in epididymal adipose tissue of high fat (HF) fed mice were further evaluated. Overnight treatment of DNJ increased cholesterol efflux mediated with ApoA1 and HDL in 3T3-L1 adipocytes. DNJ increased the protein expression of ABCA1 gene and enhanced the nuclear presence of LXRα, the transcription factor for ABCA1 in 3T3-L1 adipocytes. DNJ supplementation for 12. weeks upregulated the expression of ABCA1 in epididymal adipose tissue in HF fed mice, however, the effect was not related to the increase in circulating HDL-cholesterol levels. Thus, DNJ appears to have a positive impact on the reverse cholesterol metabolism by activating the ABCA1-LXRα pathway and enhancing the cholesterol efflux. DNJ may decrease the risk of metabolic disorders related to abnormal regulation of reverse cholesterol transport.

Original languageEnglish
Pages (from-to)692-699
Number of pages8
JournalJournal of Functional Foods
Volume7
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

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1-Deoxynojirimycin
deoxynojirimycin
adipocytes
Adipocytes
Cholesterol
cholesterol
ATP Binding Cassette Transporter 1
Fats
adipose tissue
Adipose Tissue
mice
lipids
Gene Expression
cholesterol metabolism
metabolic diseases
high density lipoprotein cholesterol
HDL Cholesterol
cytotoxicity
Transcription Factors
transcription factors

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

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title = "Effect of 1-deoxynojirimycin on cholesterol efflux through ABCA1-LXRα pathway in 3T3-L1 adipocytes",
abstract = "We aimed to investigate whether 1-deoxynojirimycin (DNJ) has effects on cholesterol efflux in adipocytes and modulates ABCA1 expression in high fat fed mice. Fully differentiated 3T3-L1 adipocytes were tested for the cytotoxicity of DNJ and cholesterol efflux into apoA1/HDL was then assessed. ABCA1 protein levels and nuclear abundance of LXRα were measured after the treatment of DNJ in differentiated 3T3-L1 adipocytes. The effects of 12. week DNJ supplementation on ABCA1 gene expression in epididymal adipose tissue of high fat (HF) fed mice were further evaluated. Overnight treatment of DNJ increased cholesterol efflux mediated with ApoA1 and HDL in 3T3-L1 adipocytes. DNJ increased the protein expression of ABCA1 gene and enhanced the nuclear presence of LXRα, the transcription factor for ABCA1 in 3T3-L1 adipocytes. DNJ supplementation for 12. weeks upregulated the expression of ABCA1 in epididymal adipose tissue in HF fed mice, however, the effect was not related to the increase in circulating HDL-cholesterol levels. Thus, DNJ appears to have a positive impact on the reverse cholesterol metabolism by activating the ABCA1-LXRα pathway and enhancing the cholesterol efflux. DNJ may decrease the risk of metabolic disorders related to abnormal regulation of reverse cholesterol transport.",
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Effect of 1-deoxynojirimycin on cholesterol efflux through ABCA1-LXRα pathway in 3T3-L1 adipocytes. / Do, Hyun Ju; Lee, Seung-Min; Kim, Young Soon; Shin, Min Jeong.

In: Journal of Functional Foods, Vol. 7, No. 1, 01.01.2014, p. 692-699.

Research output: Contribution to journalArticle

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