Effect of areca nut on helicobacter pylori-induced gastric diseases in mice

Jinwook Lee, Niluka D. Gunawardhana, Sungil Jang, Yun Hui Choi, Rasika P. Illeperuma, Aeryun Kim, Hanfu Su, Youngmin A. Hong, Ji Hye Kim, Jinmoon Kim, Da Woon Jung, In Ho Cha, Eun Jung Bak, Jeong Heon Cha

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Abstract

Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been identified. The aim of this study was to investigate the effect of AN on gastric diseases using a mouse model for Helicobacter pylori infection. We studied four groups of mice: those fed a normal diet (ND), those fed a diet containing 2.5% AN (AD), those fed ND and infected with H. pylori PMSS1 strain (ND/HP), and those fed AD and infected with H. pylori PMSS1 strain (AD/HP). Food intake and body weight were monitored weekly during the experiments. At 10 weeks, the mice were sacrificed, and the stomach weight, H. pylori colonization, and gastric inflammation were evaluated. The stomach weight had increased significantly in the ND/HP and AD/HP groups along with increases in H. pylori colonization; however, there was no significant difference between these two groups with respect to stomach weight and colonization. On histological grading, mononuclear cell infiltration was severer in the AD/HP group than in the ND/HP group. These data suggest that chronic gastric inflammation was aggravated by AN treatment in the mice with H. pyloriinduced gastric lesions. Furthermore, as previously suggested, this animal model is useful to determine the effect of potential carcinogens on gastric lesions induced by H. pylori infection.

Original languageEnglish
Pages (from-to)1817-1823
Number of pages7
JournalJournal of microbiology and biotechnology
Volume26
Issue number10
DOIs
Publication statusPublished - 2016 Oct

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Lee, J., Gunawardhana, N. D., Jang, S., Choi, Y. H., Illeperuma, R. P., Kim, A., Su, H., Hong, Y. A., Kim, J. H., Kim, J., Jung, D. W., Cha, I. H., Bak, E. J., & Cha, J. H. (2016). Effect of areca nut on helicobacter pylori-induced gastric diseases in mice. Journal of microbiology and biotechnology, 26(10), 1817-1823. https://doi.org/10.4014/jmb.1606.06043