Effect of centrifugal force on cellular activity of osteoblastic MC3T3-E1 cells in vitro.

D. H. Lee, Jongchul Park, H. Suh

Research output: Contribution to journalArticle

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Abstract

The effects of centrifugal force on growth and differentiation of osteoblastic cells cultured in alpha-MEM containing 1% Fetal bovine serum were investigated by assays of DNA synthesis, alkaline phosphatase activity and osteocalcin-production in osteoblastic MC3T3-E1 cells. Centrifugation of the cells in low concentrations (1%) of fetal bovine serum caused a 1.9-fold increase of [3H]thymidine incorporation on day 3 from the start of centrifugation, and gradually decreased with culture up to day 9. Alkaline phosphatase activity was not affected by centrifugal force until day 5, and increased rapidly after day 7. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. These results suggest that centrifugal force stimulates the proliferation of osteoblastic cells through an autocrine secretion of some diffusable growth- promoting activity. Additional centrifugation of the cells also slightly stimulated alkaline phosphatase activity, although this did not directly influence the cell's osteocalcin-production activity.

Original languageEnglish
Pages (from-to)405-410
Number of pages6
JournalYonsei medical journal
Volume42
Issue number4
DOIs
Publication statusPublished - 2001 Jan 1

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Centrifugation
Alkaline Phosphatase
Osteocalcin
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
DNA
Serum
Thymidine
Protein Kinase C
Cell Differentiation
Cultured Cells
Cell Proliferation
In Vitro Techniques
Growth

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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Effect of centrifugal force on cellular activity of osteoblastic MC3T3-E1 cells in vitro. / Lee, D. H.; Park, Jongchul; Suh, H.

In: Yonsei medical journal, Vol. 42, No. 4, 01.01.2001, p. 405-410.

Research output: Contribution to journalArticle

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