Effect of chlorophyllin on normothermic storage of human periodontal ligament cells

Won Gyun Chung, Eun Ju Lee, Seung Jong Lee, Seung Ae Lee, Jin Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The purpose of the present study was to evaluate whether chlorophyllin could serve as an effective constituent of a storage medium to enhance the human periodontal ligament (PDL) cell viability. Freshly isolated PDL cells from premolars extracted from healthy people were stored at 37°C for 6 h in various solutions: F-medium and Hank's balanced salt solution (HBSS), supplemented with chlorophyllin. From MTT viability assays, the highest cell viability was found in the PDL cells stored in HBSS supplemented with 500 nM chlorophyllin, and the chlorophyllin-treated cells showed a dose-dependent response to concentration. Additionally, the results from flow cytometry showed that 77 to 80% of the PDL cells were in the G0/G1 phases of the cell cycle, which suggested that most were in a stable stage. These result showed that HBSS, supplemented with chlorophyllin, may be a useful solution for preserving the viability of PDL cells.

Original languageEnglish
Pages (from-to)399-402
Number of pages4
JournalJournal of endodontics
Volume30
Issue number6
DOIs
Publication statusPublished - 2004 Jun

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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