Effect of d-allose on prostate cancer cell lines: Phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry

Rae Ung Jeong, Sangsoo Lim, Myoung Ok Kim, Myeong Hee Moon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

d-Allose, a rare, naturally occurring monosaccharide, is known to exert anti-proliferative effects on cancer cells. The effects of d-allose on the cellular membranes of hormone-refractory prostate cancer cell line (DU145), hormone-sensitive prostate cancer cell line (LNCaP), and normal prostate epithelial cells (PrEC) were studied at the molecular level by phospholipid (PL) profiling using a shotgun lipidomic method. The molecular structures of 85 PL species including 23 phosphatidylcholines, 12 phosphatidylethanolamines (PEs), 11 phosphatidylserines (PSs), 16 phosphatidylinositols, 9 phosphatidic acids (PAs), and 14 phosphatidylglycerols (PGs) were identified by data-dependent collision-induced dissociation of nanoflow liquid chromatography-tandem mass spectrometry, and the PL amounts were quantified. The addition of d-allose to prostate cancer cell lines during their growth phases had negligible or decreased effects on the relative regulation of PL species, but several new PS molecules (two for DU145 and three for LNCaP) emerged. In contrast, experiments on the PrEC cell line revealed that some high abundant species (14:0/14:0-PE, 16:2/16:0-PG, and 20:6/18:1-PA) showed significant increases in concentration. These findings support a mechanism for the anti-proliferative effect of d-allose on prostate cancer cell lines that involves the induction of programmed cell death since PS molecules are known to induce apoptosis. Principal component analysis was carried out to examine differences in PL distributions among the three cell lines promoted by d-allose.

Original languageEnglish
Pages (from-to)689-698
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume401
Issue number2
DOIs
Publication statusPublished - 2011 Aug 1

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Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Phospholipids
Prostatic Neoplasms
Cells
Cell Line
Phosphatidylserines
Phosphatidylethanolamines
Phosphatidylglycerols
Phosphatidic Acids
Prostate
Epithelial Cells
Hormones
Monosaccharides
Firearms
Molecules
Phosphatidylinositols
Principal Component Analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

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abstract = "d-Allose, a rare, naturally occurring monosaccharide, is known to exert anti-proliferative effects on cancer cells. The effects of d-allose on the cellular membranes of hormone-refractory prostate cancer cell line (DU145), hormone-sensitive prostate cancer cell line (LNCaP), and normal prostate epithelial cells (PrEC) were studied at the molecular level by phospholipid (PL) profiling using a shotgun lipidomic method. The molecular structures of 85 PL species including 23 phosphatidylcholines, 12 phosphatidylethanolamines (PEs), 11 phosphatidylserines (PSs), 16 phosphatidylinositols, 9 phosphatidic acids (PAs), and 14 phosphatidylglycerols (PGs) were identified by data-dependent collision-induced dissociation of nanoflow liquid chromatography-tandem mass spectrometry, and the PL amounts were quantified. The addition of d-allose to prostate cancer cell lines during their growth phases had negligible or decreased effects on the relative regulation of PL species, but several new PS molecules (two for DU145 and three for LNCaP) emerged. In contrast, experiments on the PrEC cell line revealed that some high abundant species (14:0/14:0-PE, 16:2/16:0-PG, and 20:6/18:1-PA) showed significant increases in concentration. These findings support a mechanism for the anti-proliferative effect of d-allose on prostate cancer cell lines that involves the induction of programmed cell death since PS molecules are known to induce apoptosis. Principal component analysis was carried out to examine differences in PL distributions among the three cell lines promoted by d-allose.",
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Effect of d-allose on prostate cancer cell lines : Phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry. / Jeong, Rae Ung; Lim, Sangsoo; Kim, Myoung Ok; Moon, Myeong Hee.

In: Analytical and Bioanalytical Chemistry, Vol. 401, No. 2, 01.08.2011, p. 689-698.

Research output: Contribution to journalArticle

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T2 - Phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry

AU - Jeong, Rae Ung

AU - Lim, Sangsoo

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