Effect of EI24 expression on the tumorigenesis of ApcMin/+ colorectal cancer mouse model

Tae Wook Nam, Song Yi Park, Jae Hoon Lee, Jae il Roh, Han Woong Lee

Research output: Contribution to journalArticle

Abstract

Etoposide-induced 2.4 kb transcript (EI24, also known as PIG8) is a p53 target gene involved in cell growth suppression and apoptosis and known to be frequently altered in human cancers. Although EI24 expression is decreased in various cancers and is associated with colorectal cancer progression and metastasis, the physiological function of EI24 in colorectal cancer is yet unclear. We generated an Ei24 conditional transgenic (Tg) mouse to study the therapeutic effects of Ei24 in vivo and evaluated whether Ei24 plays a role of a tumor suppressor using Ei24 Tg mouse crossed with ApcMin/+ mouse, which develops multiple intestinal adenomas. The overexpression of Ei24 failed to cause any notable difference in the number of polyps, lengths of the intestine and spleen, and survival rate between ApcMin/+ and ApcMin/+ Ei24 Tg mice. Ei24 plays no significant role in colon cancer caused by the substitutional mutation of Apc in mice. Therefore, our result dismisses the hypothesized direct link between ApcMin/+ mutation and Ei24 expression in colorectal cancer model.

Original languageEnglish
Pages (from-to)1087-1092
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume514
Issue number4
DOIs
Publication statusPublished - 2019 Jul 5

Fingerprint

Cell growth
Etoposide
Transgenic Mice
Tumors
Colorectal Neoplasms
Carcinogenesis
Genes
Apoptosis
Neoplasms
Mutation
p53 Genes
Therapeutic Uses
Polyps
Adenoma
Colonic Neoplasms
Intestines
Spleen
Neoplasm Metastasis
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nam, Tae Wook ; Park, Song Yi ; Lee, Jae Hoon ; Roh, Jae il ; Lee, Han Woong. / Effect of EI24 expression on the tumorigenesis of ApcMin/+ colorectal cancer mouse model. In: Biochemical and Biophysical Research Communications. 2019 ; Vol. 514, No. 4. pp. 1087-1092.
@article{532c3eb36f7f4c5b89c359b6eaec0718,
title = "Effect of EI24 expression on the tumorigenesis of ApcMin/+ colorectal cancer mouse model",
abstract = "Etoposide-induced 2.4 kb transcript (EI24, also known as PIG8) is a p53 target gene involved in cell growth suppression and apoptosis and known to be frequently altered in human cancers. Although EI24 expression is decreased in various cancers and is associated with colorectal cancer progression and metastasis, the physiological function of EI24 in colorectal cancer is yet unclear. We generated an Ei24 conditional transgenic (Tg) mouse to study the therapeutic effects of Ei24 in vivo and evaluated whether Ei24 plays a role of a tumor suppressor using Ei24 Tg mouse crossed with ApcMin/+ mouse, which develops multiple intestinal adenomas. The overexpression of Ei24 failed to cause any notable difference in the number of polyps, lengths of the intestine and spleen, and survival rate between ApcMin/+ and ApcMin/+ Ei24 Tg mice. Ei24 plays no significant role in colon cancer caused by the substitutional mutation of Apc in mice. Therefore, our result dismisses the hypothesized direct link between ApcMin/+ mutation and Ei24 expression in colorectal cancer model.",
author = "Nam, {Tae Wook} and Park, {Song Yi} and Lee, {Jae Hoon} and Roh, {Jae il} and Lee, {Han Woong}",
year = "2019",
month = "7",
day = "5",
doi = "10.1016/j.bbrc.2019.04.186",
language = "English",
volume = "514",
pages = "1087--1092",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

Effect of EI24 expression on the tumorigenesis of ApcMin/+ colorectal cancer mouse model. / Nam, Tae Wook; Park, Song Yi; Lee, Jae Hoon; Roh, Jae il; Lee, Han Woong.

In: Biochemical and Biophysical Research Communications, Vol. 514, No. 4, 05.07.2019, p. 1087-1092.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of EI24 expression on the tumorigenesis of ApcMin/+ colorectal cancer mouse model

AU - Nam, Tae Wook

AU - Park, Song Yi

AU - Lee, Jae Hoon

AU - Roh, Jae il

AU - Lee, Han Woong

PY - 2019/7/5

Y1 - 2019/7/5

N2 - Etoposide-induced 2.4 kb transcript (EI24, also known as PIG8) is a p53 target gene involved in cell growth suppression and apoptosis and known to be frequently altered in human cancers. Although EI24 expression is decreased in various cancers and is associated with colorectal cancer progression and metastasis, the physiological function of EI24 in colorectal cancer is yet unclear. We generated an Ei24 conditional transgenic (Tg) mouse to study the therapeutic effects of Ei24 in vivo and evaluated whether Ei24 plays a role of a tumor suppressor using Ei24 Tg mouse crossed with ApcMin/+ mouse, which develops multiple intestinal adenomas. The overexpression of Ei24 failed to cause any notable difference in the number of polyps, lengths of the intestine and spleen, and survival rate between ApcMin/+ and ApcMin/+ Ei24 Tg mice. Ei24 plays no significant role in colon cancer caused by the substitutional mutation of Apc in mice. Therefore, our result dismisses the hypothesized direct link between ApcMin/+ mutation and Ei24 expression in colorectal cancer model.

AB - Etoposide-induced 2.4 kb transcript (EI24, also known as PIG8) is a p53 target gene involved in cell growth suppression and apoptosis and known to be frequently altered in human cancers. Although EI24 expression is decreased in various cancers and is associated with colorectal cancer progression and metastasis, the physiological function of EI24 in colorectal cancer is yet unclear. We generated an Ei24 conditional transgenic (Tg) mouse to study the therapeutic effects of Ei24 in vivo and evaluated whether Ei24 plays a role of a tumor suppressor using Ei24 Tg mouse crossed with ApcMin/+ mouse, which develops multiple intestinal adenomas. The overexpression of Ei24 failed to cause any notable difference in the number of polyps, lengths of the intestine and spleen, and survival rate between ApcMin/+ and ApcMin/+ Ei24 Tg mice. Ei24 plays no significant role in colon cancer caused by the substitutional mutation of Apc in mice. Therefore, our result dismisses the hypothesized direct link between ApcMin/+ mutation and Ei24 expression in colorectal cancer model.

UR - http://www.scopus.com/inward/record.url?scp=85065524056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065524056&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2019.04.186

DO - 10.1016/j.bbrc.2019.04.186

M3 - Article

VL - 514

SP - 1087

EP - 1092

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -