Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice

Soo Min Cho, Jin A. Park, Na Hyun Kim, Dong Soon Kim, Dan Zhang, Hee Yi, Hee Jung Cho, Ja Kyung Kim, Dong Ki Lee, Jin Suk Kim, Ho Chul Shin

Research output: Contribution to journalArticle

Abstract

The present study investigated the preventive effect of w-3 fatty acids against cholesterol gallstone (CG) formation. CG formation was induced in C57BL/6J mice using a lithogenic diet (LD). The mice were divided into four treatment groups: i) LD, ii) LD plus eicosapentaenoic acid (EPA), iii) LD plus docosahexaenoic acid (DHA) and iv) LD plus EPA plus DHA. Subsequent to feeding the mice the LD for four weeks, EPA and/or DHA (70 mg/kg/day) were orally administered for eight weeks. The mice in the EPA treatment groups exhibited significantly less gallstone formation than those in the LD group. By contrast, DHA treatment only slightly suppressed gallstone formation. The expression of mucin 2, 5AC, 5B and 6 was significantly decreased in the gallbladders of mice in the EPA groups (70.90%) and the LD plus DHA group (30.50%), compared with that in the mice in the LD group. In addition, the mRNA expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase was significantly decreased in the livers of mice in the EPA treatment group compared with that in the livers of mice in the LD group. In conclusion, EPA was found to have a dominant anti-lithogenic effect in C57BL/6J mice.

Original languageEnglish
Pages (from-to)362-366
Number of pages5
JournalMolecular Medicine Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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  • Cite this

    Cho, S. M., Park, J. A., Kim, N. H., Kim, D. S., Zhang, D., Yi, H., Cho, H. J., Kim, J. K., Lee, D. K., Kim, J. S., & Shin, H. C. (2015). Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice. Molecular Medicine Reports, 11(1), 362-366. https://doi.org/10.3892/mmr.2014.2687